EMAGE mouse gene expression spatial database

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EMAGE:20184

Ttn titin ( MGI:98864)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012439. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012439_01	euxassay_012439_02	euxassay_012439_03	euxassay_012439_04

euxassay_012439_05	euxassay_012439_06	euxassay_012439_07	euxassay_012439_08	euxassay_012439_09

euxassay_012439_10	euxassay_012439_11	euxassay_012439_12	euxassay_012439_13	euxassay_012439_14

euxassay_012439_15	euxassay_012439_16	euxassay_012439_17	euxassay_012439_18	euxassay_012439_19

euxassay_012439_20	euxassay_012439_21	euxassay_012439_22	euxassay_012439_23	euxassay_012439_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20184_wholemount_strong.wlz

20184_wholemount_moderate.wlz

20184_wholemount_weak.wlz

20184_wholemount_possible.wlz

20184_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20184_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

upper leg muscle

strong

regional

strong expression: see section 03 04 05 06 07 08 23 24

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 18 19 20 21 22 23 24

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02

not examined

regional

not examined expression: see section 06 07 08

tarsus

strong

regional

strong expression: see section 06 07 08 21 22 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 04

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

extrinsic ocular muscle

strong

regional

strong expression: see section 02 03 04 05 06 07 18 19 20 21 22 23 24

heart atrium

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18 moderate expression: see section 06 07 19 20 21 22 23

heart ventricle

strong

regional

strong expression: see section 08 09 10 11 12 13 14 15 16 17 18

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17

tail mesenchyme

strong

regional

strong expression: see section 12 13 14 15 16 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38075
Entity Detected:	Ttn, titin ( MGI:98864)
Sequence:	sense strand is shown >T38075 GGAATACGAAATCTCAAGACGGGCTCAAAGGGAATCATCCTCCTCCGTGTCCAGACTACTGAGACGCCGT CGCTCCTTGTCTCCAACTTATATTGAGTTAATGAGACCAGTGTCTGAGCTGATTCGGTCCCATCCACGAC CTGCTGAGGAATATGAAGATGATGCAGAAAGAAGGTCTCCTACTCCAGAGAGAACTCGCCCACGTTCTCC CAGCCCTGTGTCTAGCGAGCGATCCCTCTCAAGATTTGAGAGGTCTGCAAGATTTGACATCTTCTCCAGG TACGAGTCCATGAAAGCTGCCTTAAAAACTCAGAAGACATCGGAAAGGAAGTATGAGGTTTTGAGTCAGC AGCCTTTCACACTGGACCATGCCCCTCGAATCACACTGCGGATGCGCTCCCACAGGGTTCCCTGTGGCCA AAACACACGCTTCATCCTAAACGTCCAGTCGAAGCCGACTGCCGAAGTTAAATGGTACCACAATGGTGTA GAACTCCAAGAGAGCAGTAAGATTCATTACACCAACACAAGTGGGGTCCTGACCTTGGAGATTCTGGACT GCCAGACTGAGGACGGTGGAACCTACCGTGCCGTGTGCACCAACTACAAAGGAGAGGCATCAGACTACGC AACCCTGGATGTAACTGGCGGAGCTTATACCACCTATGCATCCCAGCGCAGAGATGAGGAGGTCCCCAAG TCAGTCTTCCCTGAACTGACAAAAACAGAGGCATATGCAGTCTCGTCGTTCAAGAGAACATCTGAGCTGG AAGCTGCTTCTTCAGTCAGGGAAGTGAAGTCACAAATGAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 76632. Forward Primer - name:076632_F_cDNA_Ttn, sequence:GGAATACGAAATCTCAAGACGG; Reverse Primer - name:076632_N_SP6_cDNA_Ttn, sequence:GTCATTTGTGACTTCACTTCCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20181 same embryo
	EMAGE:20182 same embryo
	EMAGE:20185 same embryo
	EMAGE:20183 same embryo
	EMAGE:20180 same embryo
	EurExpress:euxassay_012439 same experiment
	MGI:4829001 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source