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EMAGE:20264

Lrrfip2 leucine rich repeat (in FLII) interacting protein 2 ( MGI:1918518)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264
"Pseudo-wholemount" of euxassay_012383. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012383_01 euxassay_012383_02 euxassay_012383_03 euxassay_012383_04
EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264
euxassay_012383_05 euxassay_012383_06 euxassay_012383_07 euxassay_012383_08 euxassay_012383_09
EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264
euxassay_012383_10 euxassay_012383_11 euxassay_012383_12 euxassay_012383_13 euxassay_012383_14
EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264 EMAGE:20264
euxassay_012383_15 euxassay_012383_16 euxassay_012383_17 euxassay_012383_18 euxassay_012383_19
EMAGE:20264 EMAGE:20264 EMAGE:20264
euxassay_012383_20 euxassay_012383_21 euxassay_012383_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20264Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20264_wholemount_strong.wlz
20264_wholemount_moderate.wlz
20264_wholemount_weak.wlz
20264_wholemount_possible.wlz
20264_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20264_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
mandible
moderate moderate
regionalmoderate expression: see section 08 09 10 21 weak expression: see section 04 06 07 11 19 20
maxilla
moderate moderate
regionalmoderate expression: see section 08 09 10 weak expression: see section 05 06 07 19 20
orbito-sphenoid
moderate moderate
regionalmoderate expression: see section 03 04 05 21 22 weak expression: see section 01 02 06
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31272
Entity Detected:Lrrfip2, leucine rich repeat (in FLII) interacting protein 2 ( MGI:1918518)
Sequence:sense strand is shown

>T31272
CCCAGACAGCACTCCCAATGGTGATGTCCATCATGAGCCTGTGGTTGGAGCCATTACTGCTGTGTCTCAG
GAAGCTGCTCAGGTCTTGGAGTCAGCAGGAGAAGGGCCACTAGATGTGAGGCTACGAAAGCTTGCTGGAG
AAAAGGACGAGCTCTTGTCACAGATTAGAAAACTGAAGCTCCAGTTAGAGGAAGAACGGCAGAAGTGTTC
CAGGAATGATGGCATGTCCGGGGACCTGGCAGGACTGCAGAACGGCTCAGACTTGCAGTTCATCGAGATG
CAGAGAGATGCCAATAGACAAATTAGTGAATACAAATTCAAGCTTTCGAAAGCAGAACAAGACATAGCTA
CCTTGGAACAAAGTATCAGCCGGCTTGAGGGGCAGGTGCTGAGGTACAAAACTGCTGCTGAGAATGCAGA
GAAAATTGAAGATGAGCTGAAAGCAGAAAGGAGGAAGCTACAGCGAGAGCTACGGACGGCACAGGACAAG
ATAGAGGAGATGGAGATGACCAACAGCCACCTGGCTAAGCGGCTAGAGAAGATGAAAGCCAACAGGACAG
CCCTTCTAGCCCAGCAGTAGGAGGCGGCCCTTCTACCTGGGTGCTGCTGAGGACCTTGCCGGGAGATGCT
GACTCTTTTGTACATTGACACAAGCCCCTTCAA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4217995), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 54968. Forward Primer - name:054968_F_IRAV49_g07_Lrrfip2, sequence:CCCAGACAGCACTCCCAA; Reverse Primer - name:054968_R_SP6_IRAV49_g07_Lrrfip2, sequence:CTTGAAGGGGCTTGTGTCA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20261 same embryo
 EMAGE:20260 same embryo
 EMAGE:20263 same embryo
 EMAGE:20259 same embryo
 EMAGE:20262 same embryo
 EurExpress:euxassay_012383 same experiment
 MGI:4826003 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS