EMAGE mouse gene expression spatial database

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EMAGE:20361

Fam81a family with sequence similarity 81, member A ( MGI:1924136)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012569. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012569_01	euxassay_012569_02	euxassay_012569_03	euxassay_012569_04

euxassay_012569_05	euxassay_012569_06	euxassay_012569_07	euxassay_012569_08	euxassay_012569_09

euxassay_012569_10	euxassay_012569_11	euxassay_012569_12	euxassay_012569_13	euxassay_012569_14

euxassay_012569_15	euxassay_012569_16	euxassay_012569_17	euxassay_012569_18	euxassay_012569_19

euxassay_012569_20	euxassay_012569_21	euxassay_012569_22	euxassay_012569_23	euxassay_012569_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20361_wholemount_strong.wlz

20361_wholemount_moderate.wlz

20361_wholemount_weak.wlz

20361_wholemount_possible.wlz

20361_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20361_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21 moderate expression: see section 03 04 05 06 12 13 14 15 16

vibrissa

moderate

regional

moderate expression: see section 04 05 06 21 22 23 24

diencephalon roof plate

moderate

regional

moderate expression: see section 13 14 15

choroid invagination

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 moderate expression: see section 12 16

medulla oblongata alar plate mantle layer

weak

regional

weak expression: see section 09

rest of cerebellum mantle layer

moderate

regional

moderate expression: see section 06 07 19

pons mantle layer

weak

regional

weak expression: see section 07 08 17

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 07 08 09 10 11 17 18 19 20 21 moderate expression: see section 03 04 05 06 12 13 14 15 16

trigeminal v ganglion

moderate

regional

moderate expression: see section 09 10 18 19 20 21 22 weak expression: see section 04 05 06 07 08

anterior naris epithelium

weak

regional

weak expression: see section 11 12 13

external naris epithelium

weak

regional

weak expression: see section 12

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 10 11 12 13 14 15 16 17 18

mandible

weak

regional

weak expression: see section 07 08 09 10 11 12 16 17 18 19

maxilla

weak

regional

weak expression: see section 07 08 09 10 11 12 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31546
Entity Detected:	Fam81a, family with sequence similarity 81, member A ( MGI:1924136)
Sequence:	sense strand is shown >T31546 GGTGGAGCAGCTGGAAGACAGGATCCTCTGTCATGAGAAAACCACAGCAGCACTGGTGGAGCATGCCTTC CGCATCAAAGATGACATCGTCAGCAGTTTGCAGAAGATGCAGAATAAAGGGGGAGGTGACCGCTTAGCCA GGCTGTTCTTGGAAGAGCATATCAGAAACATAACCGCCATTGTGAAGCAACTGAATCGGGATATTGAGGT TCTCCAGGAGCAGATCCGTGCCAGGGACAACATCAGCTATGGAACTAATTCTGCCTTAAAGACGCTGGAG ATGCGCCAGCTCTCTGGCTTGGGAGATCTCCGAGGAAGAGTTGCAAGGTGCGACGCCAGCATAGCCAGGC TCTCTGCCGAGCATAAGTCGACCTACGAGGGACTCCAGCACTTGAACAAGGAACAGCAAGCAGCCAAGCT TATCCTGGAAACAAAAATCAAAGACGCAGAAGGACAGATTTCTCAGCTTTTGAGCAGAGTGGACTTGTCC ATCTCAGAGCAGAGCACCAAACTGAAGATGTCCCACAGGGACAGCAACCACCAGCTCCAGCTCCTGGACA CTAAATTTAAAGGCACCGTTGAAGAGCTCAGCAACCAGATTTTGTCTGCACGGAGTTGGCTGCAACAAGA ACAGGAGCGGATAGAGAAAGAACTTCTGCAGAAAATAGACCATCTCTCCTTGATTGTTAAGGAAAACAGT GGAGCCAATGAAAGAGACGTAGAGAAGAAACTCAGCCAGATGTCGGCCAGGCTTGACAAAATCGAAGAGA GTCAGAAGAGGAATGCTGAAGGACAGAGAAAGCCGGACGAGGAGAAGGTGCAC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:5324986), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 31441. Forward Primer - name:031441_F_IRAV67-70_L16_6430514L14Rik, sequence:GGTGGAGCAGCTGGAAGA; Reverse Primer - name:031441_R_SP6_IRAV67-70_L16_6430514L14Rik, sequence:CGTGCACCTTCTCCTCGT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20359 same embryo
	EMAGE:20360 same embryo
	EMAGE:20358 same embryo
	EMAGE:20363 same embryo
	EMAGE:20362 same embryo
	EurExpress:euxassay_012569 same experiment
	MGI:4824760 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source