EMAGE mouse gene expression spatial database

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EMAGE:20404

Gale galactose-4-epimerase, UDP ( MGI:1921496)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_012604. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_012604_01	euxassay_012604_02	euxassay_012604_03	euxassay_012604_04

euxassay_012604_05	euxassay_012604_06	euxassay_012604_07	euxassay_012604_08	euxassay_012604_09

euxassay_012604_10	euxassay_012604_11	euxassay_012604_12	euxassay_012604_13	euxassay_012604_14

euxassay_012604_15	euxassay_012604_16	euxassay_012604_17	euxassay_012604_18	euxassay_012604_19

euxassay_012604_20	euxassay_012604_21	euxassay_012604_22	euxassay_012604_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20404_wholemount_strong.wlz

20404_wholemount_moderate.wlz

20404_wholemount_weak.wlz

20404_wholemount_possible.wlz

20404_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20404_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 13 14 15 16 17 18 19 20 22 23 weak expression: see section 01 21

hindlimb digit 1 metatarsal

moderate

regional

moderate expression: see section 19

hindlimb digit 1 phalanx

weak

regional

weak expression: see section 18

hindlimb digit 2 metatarsal

moderate

regional

moderate expression: see section 19 weak expression: see section 01

hindlimb digit 2 phalanx

moderate

regional

moderate expression: see section 19 weak expression: see section 01 18

hindlimb digit 3 metatarsal

moderate

regional

moderate expression: see section 19 weak expression: see section 01

hindlimb digit 3 phalanx

moderate

regional

moderate expression: see section 19 weak expression: see section 01 18

hindlimb digit 4 metatarsal

moderate

regional

moderate expression: see section 19 weak expression: see section 01

hindlimb digit 4 phalanx

moderate

regional

moderate expression: see section 19 weak expression: see section 01 18

hindlimb digit 5 metatarsal

weak

regional

weak expression: see section 18

hindlimb digit 5 phalanx

weak

regional

weak expression: see section 01 18

otic capsule

moderate

regional

moderate expression: see section 06 07 08 15 16 17

nasal septum

moderate

regional

moderate expression: see section 09 10 11 12 13 14

rectum

moderate

regional

moderate expression: see section 09

mandible

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 10 14 15 16 17 18 19 20 21 22

meckel's cartilage

moderate

regional

moderate expression: see section 11 12

maxilla

moderate

regional

moderate expression: see section 04 05 06 07 08 16 17 18 19 20 21

thyroid cartilage

moderate

regional

moderate expression: see section 10 weak expression: see section 11 12 13

axial skeleton

moderate

regional

moderate expression: see section 08 09 10 11 weak expression: see section 12 13

basisphenoid bone

moderate

regional

moderate expression: see section 01 23

exoccipital bone

moderate

regional

moderate expression: see section 03 04 05 06 19 20 21 22

temporal bone petrous part

moderate

regional

moderate expression: see section 01 02 03 04 05 06 19 20 21 22 23

orbito-sphenoid

strong

regional

strong expression: see section 17 moderate expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

viscerocranium

strong

regional

Expression in the turbinate bone.

clavicle

moderate

regional

moderate expression: see section 08 15 16 weak expression: see section 17 18

sternum

moderate

regional

moderate expression: see section 11 12

pelvic girdle skeleton

moderate

regional

moderate expression: see section 14 weak expression: see section 06 12 13

axial skeleton tail region

moderate

regional

moderate expression: see section 09 10

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31599
Entity Detected:	Gale, galactose-4-epimerase, UDP ( MGI:1921496)
Sequence:	sense strand is shown >T31599 TTGGAGTGAGGCCCTGAGCAGGAAGCCTCGGCCACCTCTGAAACTCTGTCTTCTAAGCAGGCGCCATGGA AAAGGTGCTGGTCACAGGTGGGGCTGGCTACATCGGCAGCCACACGGTATTGGAGCTGCTGGAGGCAGGC TACTCACCTGTGGTCATTGACAACTTCCATAACGCCATTCGTGGAGAAGACTCCATGCCTGAGAGCCTGC GCCGGGTCCAGGAGTTGACAGGCCGCTCTGTGGAGTTTGAGGAGATGGACATCTTGGACCAGGCAGCCCT ACAGCACCTCTTTAAGAAGCACAGCTTTAAGGCCGTCATCCACTTTGCTGGCCTCAAGGCTGTGGGCGAG TCAGTGCAGAAGCCTCTGGACTACTATAGAGTTAACTTAACAGGGACCATCCAGCTTCTAGAGATCATGA GGGCCCACGGAGTGAAGAACCTGGTGTTCAGCAGCTCAGCCACCGTGTACGGGAACCCCCAGTACCTGCC TCTGGATGAGGCCCACCCCACGGGGGGCTGTACCAACCCCTACGGCAAGTCCAAGTTCTTCATCGAGGAG ATGATCCGGGACCTGTGCCGGGCAGACACGGCCTGGAACGCCGTGCTGCTTCGGTACTTCAATCCCATAG GCGCCCACGCCTCTGGGCGCATCGGTGAAGATCCTCAGGGTATTCCCAACAACCTCATGCCCTACGTCTC CCAGGTGGCAATCGGGCGACGAGAGGCCCTGAATGTCTTTGGTGATGACTATGCTACAGAGGACGGGACA GGTGTGAGGGATTACATTCACGTGGTGGACCTGGCGAAGGGCCATATAGCAGCCTTGAAGAAGCTGAAGG AGCAATGTGGTTGCCGGACCT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4224021), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 29978. Forward Primer - name:029978_F_IRAV77-80_D14_Gale, sequence:TTGGAGTGAGGCCCTGAG; Reverse Primer - name:029978_R_SP6_IRAV77-80_D14_Gale, sequence:TAGGTCCGGCAACCACAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20405 same embryo
	EurExpress:euxassay_012604 same experiment
	MGI:4824977 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source