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EMAGE:20412

Fam176b family with sequence similarity 176, member B ( MGI:1922063)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412
"Pseudo-wholemount" of euxassay_012622. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_012622_01 euxassay_012622_02 euxassay_012622_03 euxassay_012622_04
EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412
euxassay_012622_05 euxassay_012622_06 euxassay_012622_07 euxassay_012622_08 euxassay_012622_09
EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412
euxassay_012622_10 euxassay_012622_11 euxassay_012622_12 euxassay_012622_13 euxassay_012622_14
EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412 EMAGE:20412
euxassay_012622_15 euxassay_012622_16 euxassay_012622_17 euxassay_012622_18 euxassay_012622_19
EMAGE:20412 EMAGE:20412 EMAGE:20412
euxassay_012622_20 euxassay_012622_21 euxassay_012622_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:20412Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
20412_wholemount_strong.wlz
20412_wholemount_moderate.wlz
20412_wholemount_weak.wlz
20412_wholemount_possible.wlz
20412_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:20412_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
cervical intervertebral disc
weak weak
regionalweak expression: see section 08
lumbar intervertebral disc
moderate moderate
regionalmoderate expression: see section 09 11
thoracic intervertebral disc
moderate moderate
regionalmoderate expression: see section 11 weak expression: see section 08
mesenchyme
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22
sublingual gland primordium
weak weak
regionalweak expression: see section 08 09 14
submandibular gland primordium
weak weak
regionalweak expression: see section 06 07 15
tongue muscle
weak weak
regionalweak expression: see section 11 12 13 14
stomach
weak weak
regionalweak expression: see section 01 02 03 04 05 06 07 08 09
hindgut
weak weak
regionalweak expression: see section 11 12 13
midgut
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16 17 18 19 20
bladder
weak weak
regionalweak expression: see section 11 12 13
metanephros
weak weak
regionalweak expression: see section 04 05 06 07 13 14 15 16 17
trachea
weak weak
regionalweak expression: see section 10 11
axial skeleton sacral region
moderate moderate
regionalmoderate expression: see section 11
tail mesenchyme
weak weak
regionalweak expression: see section 10 11 14 15
axial skeleton tail region
weak weak
regionalweak expression: see section 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T31607
Entity Detected:Fam176b, family with sequence similarity 176, member B ( MGI:1922063)
Sequence:sense strand is shown

>T31607
CGATCCAGTGAGGCGAACCGCTCCCTCGCCCCACCACCACCCCAGGGTGGAGGAGCTACGCGAGGAGCGA
GCGCCTTGGGCTCTGGAAGACCTGACCTGGGTGACCTCGCAGGCTGACAGGCGAGCTGCATCCCAGCAGC
ATGGATGCCCCAAGAAGGGACATGGAGCTACTCAGCAACAGCCTGGCAGCCTATGCGCACATCAGAGCTA
ACCCCGAGAGCTTCGGCCTCTACTTCGTGCTTGGCGTCTGCTTCGGCCTGCTGCTGACCCTATGCCTGCT
GGTCATCAGCATCTCCTGCGCGCCTCGCTCGCGACCCCGGACCCCTGCTCCGCGCCGGGACCCCCGGAGC
AGCACCCTAGAGCCGGAAGACGAGGACGATGAGGAAGATGAGGACACCATGACGAGGCTGGGTCCCGATG
ACACGCTGCAGGGCCAGGAGCTGTCCACAGAGCCCGACGGGCCCCTTAGTGTCAATGTCTTCACGTCAGC
AGAGGAGCTAGAGCGGGCACAGCGGCTGGAGGAGCGTGAACGAATCCTGCGGGAGATCTGGCGCACTGGG
CAGCCAGACCTGCTGGGCAGTGGCACGCTCGGGCCCGGAGCCACGGCCACGCTGGGACGAATGCACTATT
ACTGACACCCGGCCCCTCAGCGTGGGGCCAGAGTACTGGATATGGGACTGCCTCAGGGCCTTCAAAAAAC
TGCCTCTTGCCCCCAAATCTCTTTGGTGCTATTGGGCGTCACATCGCCGCCTGCTAAGCACTCTTCATAG
GGCAGGTGTGGGTGGGAAAAGAATGCCAGCTCCCACCTTCTCCCGA
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3964022), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 28759. Forward Primer - name:028759_F_IRAV77-80_J17_2610027C15Rik, sequence:CGATCCAGTGAGGCGAAC; Reverse Primer - name:028759_R_SP6_IRAV77-80_J17_2610027C15Rik, sequence:ATCGGGAGAAGGTGGGAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:20413 same embryo
 EMAGE:20414 same embryo
 EurExpress:euxassay_012622 same experiment
 MGI:4824724 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS