EMAGE mouse gene expression spatial database

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EMAGE:20587

3110082D06Rik RIKEN cDNA 3110082D06 gene ( MGI:1920485)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_014092. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_014092_01	euxassay_014092_02	euxassay_014092_03	euxassay_014092_04

euxassay_014092_05	euxassay_014092_06	euxassay_014092_07	euxassay_014092_08	euxassay_014092_09

euxassay_014092_10	euxassay_014092_11	euxassay_014092_12	euxassay_014092_13	euxassay_014092_14

euxassay_014092_15	euxassay_014092_16	euxassay_014092_17	euxassay_014092_18	euxassay_014092_19

euxassay_014092_20	euxassay_014092_21	euxassay_014092_22	euxassay_014092_23	euxassay_014092_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20587_wholemount_strong.wlz

20587_wholemount_moderate.wlz

20587_wholemount_weak.wlz

20587_wholemount_possible.wlz

20587_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20587_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

interdigital region between hindlimb digits 1 and 2

moderate

regional

moderate expression: see section 07

interdigital region between hindlimb digits 2 and 3

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09

interdigital region between hindlimb digits 3 and 4

moderate

regional

moderate expression: see section 07 weak expression: see section 08 09

interdigital region between hindlimb digits 4 and 5

moderate

regional

moderate expression: see section 07 weak expression: see section 08 24

foot mesenchyme

weak

regional

weak expression: see section 09

diencephalon lateral wall mantle layer

moderate

single cell

moderate expression: see section 10 13

telencephalon mantle layer

moderate

single cell

moderate expression: see section 20 weak expression: see section 07 19

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 15 weak expression: see section 08 09 10 11 12 14 16 17

rest of cerebellum mantle layer

moderate

single cell

moderate expression: see section 15 weak expression: see section 16 17 18 19 20

pons mantle layer

moderate

single cell

moderate expression: see section 15 weak expression: see section 16 17 18

ventral grey horn

weak

single cell

weak expression: see section 08 09 10 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T40272
Entity Detected:	3110082D06Rik, RIKEN cDNA 3110082D06 gene ( MGI:1920485)
Sequence:	sense strand is shown >T40272 ACACAAGACCTCATAGGGGAGAAGTGGGAGAATGAGTTCTGTAAGCTTATGAGGAAGCTCCAGGAGGAGC ATCAAGACCTCCAACTGTACTCTCTGGCATCTTTTAGCCTCTGGAGAGACTTTCATAAGACCAGCATCCT GACCAGAAGCAAGGTCCTGGTGAGCCTAGTGCTGATCCTGACCACGGCCACCCTGTCCAGCTCCATGAAG GACTGCTTGCGCAGTAAGCCCTTCCTGGGCCTCCTGGGGGTGCTCACGGTCTGCATTTCCATCGCCACTG CAGCCGGGATATTCTTCATCACTGATGGGAAGTACAACTCAACCCTGCTGGGAATCCCTTTCTTCGCCAT GGGTCATGGAACTAAAGGAGTATTTGAGCTTCTGTCTGGATGGCGGAGAACCAAAGAGAATTTACCCTTC AAAGACAGGGTAGCAGATGCCTACTCTGATGTGATGGTCACCTACACCATGACCAGTTCCCTGTACTTCA TCACATTTGGTATGGGTGCCAGCCCCTTCACAAACATAGAGGCCGTGAAGATCTTCTGTCAGAATATGTG TGTCTCTATTCTGTTGAACTACTTCTACATTTTCTCTTTCTTTGGCTCTTGCCTGGTCTTTGCTGGACAA CTTGAGCAAAATCGA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 85421. Forward Primer - name:085421_F_cDNA_3110082D06Rik, sequence:ACACAAGACCTCATAGGGGAGA; Reverse Primer - name:085421_N_SP6_cDNA_3110082D06Rik, sequence:TCGATTTTGCTCAAGTTGTCC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20583 same embryo
	EMAGE:20588 same embryo
	EMAGE:20584 same embryo
	EMAGE:20586 same embryo
	EMAGE:20585 same embryo
	EurExpress:euxassay_014092 same experiment
	MGI:4822703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source