EMAGE mouse gene expression spatial database

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EMAGE:20636

Pou4f1 POU domain, class 4, transcription factor 1 ( MGI:102525)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013839. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013839_01	euxassay_013839_02	euxassay_013839_03	euxassay_013839_04

euxassay_013839_05	euxassay_013839_06	euxassay_013839_07	euxassay_013839_08	euxassay_013839_09

euxassay_013839_10	euxassay_013839_11	euxassay_013839_12	euxassay_013839_13	euxassay_013839_14

euxassay_013839_15	euxassay_013839_16	euxassay_013839_17	euxassay_013839_18	euxassay_013839_19

euxassay_013839_20	euxassay_013839_21	euxassay_013839_22	euxassay_013839_23	euxassay_013839_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20636_wholemount_strong.wlz

20636_wholemount_moderate.wlz

20636_wholemount_weak.wlz

20636_wholemount_possible.wlz

20636_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20636_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 09 10 11 12 13

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 08 09 12 14 15 17 18 moderate expression: see section 10 11 16

pons mantle layer

strong

regional

strong expression: see section 15 16 17 18

midbrain mantle layer

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 moderate expression: see section 18

facial vii ganglion

strong

regional

strong expression: see section 04 05 19 20

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 07 18

trigeminal v ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 16 17 18 19 20 21

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 06 07 08 17 18 19

dorsal grey horn

strong

regional

strong expression: see section 08 09 moderate expression: see section 10 11 12 14 15 weak expression: see section 13

dorsal root ganglion

strong

regional

strong expression: see section 07 08 12 13 14 17 18 19 moderate expression: see section 09 10 11 15 16

neural retina

strong

regional

strong expression: see section 02 03 22 23 moderate expression: see section 01 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38789
Entity Detected:	Pou4f1, POU domain, class 4, transcription factor 1 ( MGI:102525)
Sequence:	sense strand is shown >T38789 TACCGGGGATAAATGTTGAGTCACAAAAACTTCAATTTGCACTATGAGTTTCCAAAATAGAAAATCATGT GTGATATTTACATACTAATAATCCAAGCTGCTGACGGATTTTAAGGAAAAATTTTTTGAGAAAGACAGTT TGAGAAAGAAAGAGAGAGAGAGAGAAATGTGGTTTAGTGAAATGCCTGCTTTGTTGTGTAGAAGATCCCC TTTGGATCTCATGTGGAGATCGGTCTGTTACTATCATTTAACCATAACTAAAGATGAAAACGGAAGAGAT AGGGGGAACACTGTTTAACAAGACTGAAATCATGCATGGCTCGAAAGGTGCAGAAAGAAATGTGATTAGG TCTCACTCTGGGTAGGCGTTGTTATTTATTTAATTATGTTGTATGTCATTGTTTGCAGCACAAAACATTC TATGCATTGAAACTGAGCACTAAACTGGGCTAGCTTTCTGGTAGACCATTTTTTTGTGGATAGTGTGATT TCACAGTCTACTGCCTGTTTCCACTGAAAACACATTATTGTAAATATTTTCTTTCATGCACAGGCCCACA CACAGTGGTTACAAACTGCCAGTGTT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 236242. Forward Primer - name:165397_F_cDNA_Pou4f1, sequence:TACCGGGGATAAATGTTGAGTC; Reverse Primer - name:165397_N_SP6_cDNA_Pou4f1, sequence:AACACTGGCAGTTTGTAACCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	MGI:4827341 same experiment
	EurExpress:euxassay_013839 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source