EMAGE mouse gene expression spatial database

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EMAGE:20642

Tagln2 transgelin 2 ( MGI:1312985)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_001884_01	euxassay_001884_02	euxassay_001884_03	euxassay_001884_04	euxassay_001884_05

euxassay_001884_06	euxassay_001884_07	euxassay_001884_08	euxassay_001884_09	euxassay_001884_10

euxassay_001884_11	euxassay_001884_12	euxassay_001884_13	euxassay_001884_14	euxassay_001884_15

euxassay_001884_16	euxassay_001884_17	euxassay_001884_18

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20642_wholemount_strong.wlz

20642_wholemount_moderate.wlz

20642_wholemount_weak.wlz

20642_wholemount_possible.wlz

20642_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20642_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex ventricular layer

strong

homogeneous

strong expression: see section 10 11 12 moderate expression: see section 05 06 07 08 09 13 14 15 16

olfactory cortex ventricular layer

moderate

regional

moderate expression: see section 10 11 12 13

telencephalon ventricular layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1188
Entity Detected:	Tagln2, transgelin 2 ( MGI:1312985)
Sequence:	sense strand is shown >T1188 TCCTCGAGNCTGTTGGCCTACTGGAGAAAACTCCAAGCCCGGCCGGGTCTTGAGCTCCACTCGCCGCTGC AGCCCCTGTCGTGCGTGCGCTCTCATCCAGCCCTCTTGGACGCTCTTTGCCATCACCACAGCTGCTCAGA ATGGCCAACAGGGGACCTTCCTACGGCCTGAGCCGAGAGGTGCAGCAGAAGATTGAGAAGCAGTACGACG CGGATCTGGAGCAGATCCTCATCCAGTGGATCACCACTCAGTGCCGTGAGGACGTGGGCCAGCCCCAGCC TGGCCGTGAGAACTTCCAGAAGTGGCTCAAGGACGGCACGGTTCTGTGCAAGCTTATTAATTCACTGTAT CCTGAGGGGCAGGCCCCAGTAAAGAAGATCCAGGCCTCTCCGATGGCCTTCAAGCAGATGGAGCAGCTCT CCCAGTTCCTGCAGGCAGCCGAGCGCTATGGCATTAACACCACGGACATCTTCCAGACTGTGGATCTCTG GGAAGGAAAGAACATGGCTTGTGTGCAGCGGACACTAATGAACCAGGGTGGGCTGGCAGTAGCCAGGGAC GATGGGCTCTTCTCTGGGGATCCCA
Notes:	The probe template was PCR amplified from IMAGE:2159706 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2159706 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20641 same embryo
	EurExpress:euxassay_001884 same experiment
	MGI:4828591 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source