EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:20647

Mpz myelin protein zero ( MGI:103177)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_013906. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_013906_01	euxassay_013906_02	euxassay_013906_03	euxassay_013906_04

euxassay_013906_05	euxassay_013906_06	euxassay_013906_07	euxassay_013906_08	euxassay_013906_09

euxassay_013906_10	euxassay_013906_11	euxassay_013906_12	euxassay_013906_13	euxassay_013906_14

euxassay_013906_15	euxassay_013906_16	euxassay_013906_17	euxassay_013906_18	euxassay_013906_19

euxassay_013906_20	euxassay_013906_21	euxassay_013906_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

20647_wholemount_strong.wlz

20647_wholemount_moderate.wlz

20647_wholemount_weak.wlz

20647_wholemount_possible.wlz

20647_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:20647_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

facial vii ganglion

moderate

regional

moderate expression: see section 05 20

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 17

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 07 17 19

glossopharyngeal ix nerve

strong

regional

strong expression: see section 06

trigeminal v nerve

strong

regional

strong expression: see section 05 06 07 08 17 18 19

dorsal root ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 10 11 12 13 14 15 16 17

brachial plexus

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 15 16 17 18 19 20 21 22

lumbosacral plexus

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 10 11 12 13 15 16 17 18 19 20 21 22

naris

strong

regional

strong expression: see section 11 12 13 14 15

nasal septum

strong

regional

strong expression: see section 13

viscerocranium

strong

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T38786
Entity Detected:	Mpz, myelin protein zero ( MGI:103177)
Sequence:	sense strand is shown >T38786 CTATGCCAAGGGACAACCTTACATCGATGAGGTGGGGGCCTTCAAAGAGCGCATCCAGTGGGTAGGGGAC CCTCGCTGGAAGGATGGCTCCATTGTCATACACAACCTAGACTACAGTGACAATGGCACTTTCACATGTG ATGTCAAAAACCCACCAGACATAGTGGGCAAGACCTCTCAGGTCACGCTCTATGTCTTTGAAAAAGTGCC CACTAGGTATGGGGTGGTGTTGGGAGCAGTGATCGGGGGCATCCTCGGGGTGGTGCTGTTGCTGCTGTTG CTCTTCTACCTGATTCGGTACTGCTGGCTGCGCAGGCAGGCTGCCCTGCAGAGAAGGCTCAGTGCCATGG AGAAGGGGAGATTTCACAAATCTTCGAAGGACTCCTCGAAGCGAGGGCGGCAGACGCCAGTGCTGTATGC CATGCTGGACCACAGCCGAAGCACCAAAGCTGCCAGTGAGAAGAAATCAAAAGGGCTGGGGGAGTCTCGC AAGGATAAGAAATAGCGGTTAGCGGGCCGGGCGGGGGGTCGGGGGTCTGCGACGGAGTCCTCCAAAGGCT CTCAGGTGGTGGTCATCGAGATGGAGCTTCGCAAAGATGAGCAGAGCTCGGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 235762. Forward Primer - name:164917_F_cDNA_Mpz, sequence:CTATGCCAAGGGACAACCTTAC; Reverse Primer - name:164917_N_SP6_cDNA_Mpz, sequence:CTCCGAGCTCTGCTCATCTTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:20648 same embryo
	EMAGE:20646 same embryo
	EurExpress:euxassay_013906 same experiment
	MGI:4826435 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source