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EMAGE:300

Wnt4 wingless-related MMTV integration site 4 ( MGI:98957)
TS20 (12 dpc)
in situ hybridisation

Data Images
EMAGE:300
Figure 6I of Miyamoto et al., 1997 [PMID:9165114] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: no stars
Notes:
Image annotations: ub - ureteric bud; pa - pretubular cell aggregates.
Expression Pattern Description
Text Annotation:
StructureLevelPatternNotes
metanephros
detected detected
regionalExpression in an increasing number of pretubular cell aggregates.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9300
Entity Detected:Wnt4, wingless-related MMTV integration site 4 ( MGI:98957)
Notes:The Wnt4 probe used in this study by Miyamoto et al., 1997 [PMID:9165114] is indicated as that used by Stark et al., 1994 [PMID:7990960] which was originally described by Parr et al., 1993 [PMID:8275860] as a "420bp SmaI-SmaI (coding and 3' untranslated) " fragment.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:12 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
General Information
Authors:Miyamoto et al., 1997 [PMID:9165114] Indexed by GXD.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9165114] Miyamoto N, Yoshida M, Kuratani S, Matsuo I, Aizawa S 1997 Defects of urogenital development in mice lacking Emx2. Development (124):1653-64
 [ doi:10.1038/372679a0] [ PMID:7990960] Stark K, Vainio S, Vassileva G, McMahon AP 1994 Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4. Nature (372):679-83
 [ PMID:8275860] Parr BA, Shea MJ, Vassileva G, McMahon AP 1993 Mouse Wnt genes exhibit discrete domains of expression in the early embryonic CNS and limb buds. Development (119):247-61
Links:MGI:2136470 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI