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EMAGE:3231

T brachyury ( MGI:98472)
TS15 (9.0 dpc)
in situ hybridisation

Data Images
EMAGE:3231
Fig 3H of Conlon et al, 1995 [PMID:7789282] . Copyright: This image is from Conlon et al., Development 121:1533-1545 (1995), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3231Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3231_wholemount_strong_3D_1.wlz
3231_wholemount_moderate_3D_1.wlz
3231_wholemount_possible_3D_1.wlz
3231_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3231_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
notochord
strong strong
homogeneousT expression was continuous in the notochord of wt embryos
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2449092
Entity Detected:T, brachyury ( MGI:98472)
Sequence:sense strand is shown

>MGI:2449092
GGCTCCGCAGAGTGACCCTTTTTCTTGGAAAAGCGGTGGCGAGAGAAGTGAAGGTGGCTGTTGGGTAGGG
AGTCAAGACTCCTGGAAGGTGGAGAGGGTGGCGGGAGGATGAGCTCGCCGGGCACAGAGAGCGCAGGGAA
GAGCCTGCAGTACCGAGTGGACCACCTGCTCAGCGCCGTGGAGAGCGAGCTGCAGGCGGGCAGCGAGAAG
GGAGACCCCACCGAACGCGAACTGCGAGTGGGCCTGGAGGAGAGCGAGCTGTGGCTGCGCTTCAAGGAGC
TAACTAACGAGATGATTGTGACCAAGAACGGCAGGAGGATGTTCCCGGTGCTGAAGGTAAATGTGTCAGG
CCTGGACCCCAATGCCATGTACTCTTTCTTGCTGGACTTCGTGACGGCTGACAACCACCGCTGGAAATAT
GTGAACGGGGAGTGGGTACCTGGGGGCAAACCAGAGCCTCAGGCGCCCAGCTGCGTCTACATCCACCCAG
ACTCGCCCAATTTTGGGGCCCACTGGATGAAGGCGCCTGTGTCTTTCAGCAAAGTCAAACTCACCAACAA
GCTCAATGGAGGGGGACAGATCATGTTAAACTCCTTGCATAAGTATGAACCTCGGATTCACATCGTGAGA
GTTGGGGGCCCGCAACGCATGATCACCAGCCACTGCTTTCCCGAGACCCAGTTCATAGCTGTGACTGCCT
ACCAGAATGAGGAGATTACAGCCCTTAAAATTAAATACAACCCATTTGCTAAAGCCTTCCTTGATGCCAA
AGAAAGAAACGACCACAAAGATGTAATGGAGGAACCGGGGGACTGCCAGCAGCCGGGGTATTCCCAATGG
GGGTGGCTTGTTCCTGGTGCTGGCACCCTCTGCCCGCCTGCCAGCTCCCACCCTCAGTTTGGAGGCTCGC
TCTCTCTCCCCTCCACACACGGCTGTGAGAGGTACCCAGCTCTAAGGAACCACCGGTCATCGCCCTACCC
CAGCCCCTATGCTCATCGGAACAGCTCTCCAACCTATGCGGACAATTCATCTGCTTGTCTGTCCATGCTG
CAGTCCCATGATAACTGGTCTAGCCTCGGAGTGCCTGGCCACACCAGCATGCTGCCTGTGAGTCATAACG
CCAGCCCACCTACTGGCTCTAGCCAGTATCCCAGTCTCTGGTCTGTGAGCAATGGTACCATCACCCCAGG
CTCCCAGACAGCTGGGGTGTCCAACGGGCTGGGAGCTCAGTTCTTTCGAGGCTCCCCTGCACATTACACA
CCACTGACGCACACGGTCTCAGCTGCCACGTCCTCGTCTTCTGGTTCTCCGATGTATGAAGGGGCTGCTA
CAGTCACAGACATTTCTGACAGCCAGTATGACACGGCCCAAAGCCTCCTCATAGCCTCGTGGACACCTGT
GTCACCCCCATCTATGTGAATTGAACTTTCCTCCATGTGCTGAGACTTGTAACAACCGGTGTCAACTGGA
TCTTCTAGGCTCAAAGTGGCAGGCTCTTGGGACAAGGGAAAAATAAATAAATAAAAGCTAGATACTAACA
ACTCCATTTTCAAATAAGAGCAATAATACATGTCCTATAATCATGTTCTACAGCCTCTTGTTTGATACCT
ACAGTAGTGATATGTGTCCTACATTATGAAGCCAAGGACAGAGAGACGGCTGTGGTCCAGTTTTTTGTGA
CTGGCAGTTAATCAGAGTCCTTTGCTAGGTAGGGTCCTATATCTTGTGTTTCTCTACAACATATATGTGA
CTTTGAAATCCTGG
nt 1 - nt 1764 of X51683.1
Notes:The T (brachyury) probe used in this study by Conlon et al, 1995 [PMID:7789282] . is indicated as that used by Herrmann, 1991 [PMID:1821859] ie. "the sequence from base 1 to base 1764 of the cDNA for the T gene, pme75 (Herrmann et al., 1990 [PMID:2154694] )."
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.0 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase
General Information
Authors:Conlon RA, Reaume AG & Rossant J (1995) [PMID:7789282] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:7789282] Conlon RA, Reaume AG, Rossant J 1995 Notch1 is required for the coordinate segmentation of somites. Development (121):1533-45
 [ PMID:1821859] Herrmann BG 1991 Expression pattern of the Brachyury gene in whole-mount TWis/TWis mutant embryos. Development (113):913-7
 [ doi:10.1038/343617a0] [ PMID:2154694] Herrmann BG, Labeit S, Poustka A, King TR, Lehrach H 1990 Cloning of the T gene required in mesoderm formation in the mouse. Nature (343):617-22
Links:MGI:1276160 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI