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EMAGE:3241

Met met proto-oncogene ( MGI:96969)
TS16 (9.75 dpc)
in situ hybridisation

Data Images
EMAGE:3241
Fig3C Brohmann et al, 2000 [PMID:10603359] . This image is from Brohmann et al., Development 127: 437-45 (2000), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotation: D - dermomyotome. Bar = 150 micrometres.
Expression Pattern Description
Spatial Annotation:
EMAGE:3241EMAGE:3241Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D context movie

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3241_voxel_strong_3D_1.wlz
3241_voxel_moderate_3D_1.wlz
3241_voxel_possible_3D_1.wlz
3241_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3241_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
regionalExpression in the migrating muscle precursor cells that had delaminated from the lateral dermomyotomal lip and moved into the forelimb.
trunk somite
detected detected
regionalWithin dermomyotome. Expression in the migrating muscle precursor cells that had delaminated from the lateral dermomyotomal lip and moved into the forelimb.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:9337
Entity Detected:Met, met proto-oncogene ( MGI:96969)
Sequence:sense strand is shown

>MGI:9337
GAATTCGGTTAGCCTCCCAAAGCTGGTAATAGATGTGCATGAAGTGGGTGTGAACTACACAGTGGCATGT
CAGCATCGCTCAAATTCAGAGATCATCTGCTGCACTACTCCTTCACTGAAACAGCTGGGCCTGCAACTCC
CCCTGAAGACCAAAGCCTTCTTCCTGTTAGACGGGATTCTTTCCAAACACTTTGATCTCACTTATGTGCA
TAATCCTGTGTTTGAGCCTTTTGAAAAGCCAGTAATGATCTCAATGGGCAATGAAAATGTAGTGGAAATT
AAGGGAAACAATATTGACCCTGAAGCAGTTAAAGGTGAAGTGTTAAAAGTTGGAAATCAGAGCTGCGAGA
GTCTCCACTGGCACTCTGGAGCTGTGTTGTGTACAGTCCCCAGTGACCTGCTCAAACTGAACAGCGAGCT
AAATATAGAGTGGAAGCAAGCAGTCTCTTCAACTGTTCTTGGAAAAGTGATCGTTCAACCGGATCAGAAT
TTTGCAGGATTGATCATTGGTGCGGTCTCAATATCAGTAGTAGTTTTGTTATTATCCGGGCTCTTCCTGT
GGATGAGAAAGAGAAAGCATAAAGATCTGGGCAGTGAATTAGTTCGCTATGACGCAAGAGTACACACTCC
TCATTTGGATAGGCTTGTAAGTGCCCGAAGTGTAAGTCCAACTACAGAGATGGTTTCAAATGAGTCTGTA
GACTACAGAGCTACTTTTCCAGAAGACCAGTTTCCCAACTCCTCTCAGAATGGAGCATGCAGACAAGTGC
AATATCCTCTGACAGACCTGTCCCCTATCCTGACGAGTGGAGACTCTGATATATCCAGCCCATTACTACA
AAATACTGTTCACATTGACCTCAGTGCTCTAAATCCAGAGCTGGTCCAAGCAGTTCAGCACGTAGTGATT
GGACCCAGCAGCCTGATTGTGCATTTCAATGAAGTCATAGGAAGAGGGCATTTTGGCTGTGTCTATCATG
GGACTTTGCTGGACAATGACGGAAAGAAAATTCACTGTGCTGTGAAATCCTTGAATAGAATCACAGATAT
AGAAGAGGTCTCCCAGTTTCTGACTGAGGGAATCATCATGAAAGACTTCAGCCATCCCAATGTTCTCTCA
CTCTTGGGAATCTGCCTGAGGAGTGAAGGGTCTCCTCTGGTGGTCCTGCCCTATATGAAGCATGGAGATC
TGCGAAATTTCATTCGAAACGAGACTCATAATCCAACTGTGAAAGATCTTATAGGATTTGGCCTTCAAGT
AGCCAAAGGCATGAAATATCTTGCCAGCAAAAAGTTTGTCCACAGAGACTTAGCTGCAAGAAACTGCATG
TTGGATGAAAAATTCACTGTCAAGGTTGCTGATTTCGGTCTTGCCAGAGACATGTACGATAAAGAGTACT
ATAGTGTCCACAACAAGACGGGTGCCAAGCTACCAGTAAAGTGGATGGCTTTAGAGAGTCTGCAAACGCA
GAAGTTCACCACCAAGTCAGATGTGTGGTCCTTTGGTGTGCTCCTCTGGGAGCTCATGACGAGAGGAGCC
CCTCCTTATCCCGACGTGAACACATTTGATATCACTATCTACCTGTTGCAAGGCAGAAGACTCTTGCAAC
CAGAATACTGTCCAGACGCCTTGTACGAAGTGATGCTAAAATGCTGGCACCCCAAAGCGGAAATGCG
nt 2298 - nt 3974 of Y00671.1
Notes:The Met (c-met) probe used in this study by Brohmann et al., 2000 [PMID:10603359] is indicated as that originally described by Sonnenberg et al. 1993 [PMID:8408200] i.e. "murine c-met cDNA (mMetl, see Fig. 1, corresponding to position 2298-3974 in the murine DNA; Iyer et al., 1990 [PMID:2085463] ; Chan et al., 1988 [PMID:2838789] ). Y00671.1 is the original sequence deposited by Chan on 03-AUG-1988 to GenBank.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 129P2/OlaHsd
Age:9.75 dpc
Theiler Stage:TS16
Mutations:Lbx1tm1Cbm/Lbx1+
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Brohmann H; Jagla K; Birchmeier C, 2000 [PMID:10603359] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10603359] Brohmann H, Jagla K, Birchmeier C 2000 The role of Lbx1 in migration of muscle precursor cells. Development (127):437-45
 [ doi:10.1083/jcb.123.1.223] [ PMID:8408200] Sonnenberg E, Meyer D, Weidner KM, Birchmeier C 1993 Scatter factor/hepatocyte growth factor and its receptor, the c-met tyrosine kinase, can mediate a signal exchange between mesenchyme and epithelia during mouse development. J Cell Biol (123):223-35
 [ PMID:2085463] Iyer A, Kmiecik TE, Park M, Daar I, Blair D, Dunn KJ, Sutrave P, Ihle JN, Bodescot M, Vande Woude GF 1990 Structure, tissue-specific expression, and transforming activity of the mouse met protooncogene. Cell Growth Differ (1):87-95
 [ PMID:2838789] Chan AM, King HW, Deakin EA, Tempest PR, Hilkens J, Kroezen V, Edwards DR, Wills AJ, Brookes P, Cooper CS 1988 Characterization of the mouse met proto-oncogene. Oncogene (2):593-9
Links:MGI:2136979 same experiment
 MGI:2136963 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI