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EMAGE:3260

Acvr1 activin A receptor, type 1 ( MGI:87911)
TS18 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:3260
Fig2E of Yoshikawa et al, 2000 [PMID:10704880] . Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00320-2] Mech Dev 91: 439-44, Yoshikawa SI; Aota S; Shirayoshi Y; Okazaki K, The ActR-I activin receptor protein is expressed in notochord, lens placode and pituitary primordium cells in the mouse embryo. Copyright 2000

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: E, eye; N, notocord; RP, Rathke's pouch. Size bar= 200um.
Expression Pattern Description
Spatial Annotation:
EMAGE:3260Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3260_wholemount_strong_3D_1.wlz
3260_wholemount_moderate_3D_1.wlz
3260_wholemount_weak_3D_1.wlz
3260_wholemount_possible_3D_1.wlz
3260_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3260_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
notochord
detected detected
homogeneous
esophagus epithelium
detected detected
regionalExpression is in the oropharyngeal epithelium
pharynx
detected detected
regionalExpression is in the oropharyngeal epithelium
branchial arch
detected detected
otocyst
detected detected
eye
detected detected
Expression is in the surface ecotoderm of the eye.
nose
detected detected
rathke's pouch
detected detected
lens pit
strong strong
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:2135883
Entity Detected:Acvr1, activin A receptor, type 1 ( MGI:87911)
Antigen:sense strand is shown

>MGI:2135883
MVDGVMILPVLMMMAFPSPSVEDEKPKVNQKLYMCVCEGLSCGNEDHCEGQQCFSSLSINDGFHVYQKGC
FQVYEQGKMTCKTPPSPGQAVECCQGDWCNRNITAQLPTKGKSFPGTQNFHLE
aa 1 - aa 123 of NP_031420.2
Notes:The antibody used in this study by Yoshikawa et al, 2000 [PMID:10704880] is described as "mAb 2A7". The region of the ACVR1 (ActR-I/ALK2) protein used as antigen in its production was the "extracellular region (Met1-Glu123) of mouse ActR-I". The antibody recognizes product in Western blot when antigen is unreduced.
Antibody Type:monoclonal
Raised In:rat
Supplier:produced in Okazaki lab
Catalogue Number:2A7
Specimen
Organism:mouse
Strain:ICR
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol/DMSO 4:1
Secondary Antibody:sheep anti-rat Ig
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Yoshikawa SI, Aota S, Shirayoshi Y, Okazaki K (2000) [PMID:10704880] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00320-2] [ PMID:10704880] Yoshikawa SI, Aota S, Shirayoshi Y, Okazaki K 2000 The ActR-I activin receptor protein is expressed in notochord, lens placode and pituitary primordium cells in the mouse embryo. Mech Dev (91):439-44
Links:MGI:2387659 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI