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EMAGE:3268

Shh sonic hedgehog ( MGI:98297)
TS12 (8.5 dpc)
in situ hybridisation

Data Images
EMAGE:3268 EMAGE:3268 EMAGE:3268
Figure 5Fof Lowe et al., 2001 [PMID:11311163] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5A of Lowe et al., 2001 [PMID:11311163] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 5I of Lowe et al., 2001 [PMID:11311163] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: s - somite, n - notochord, nf - neural fold, hb - hindbrain, fb - forebrain, pc - prechordal plate. The approximate section planes of I and F are shown on the wholemount embryo in A. Image annotations: hb - hindbrain; fb - forebrain; n - notochord; pc - prechordal plate; s - somite. Images F and I are sections taken from the embryo shown in Image A at the levels indicated.
Expression Pattern Description
Spatial Annotation:
EMAGE:3268EMAGE:3268EMAGE:3268Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3268_voxel_moderate_3D_1.wlz
3268_voxel_notDetected_3D_1.wlz
3268_voxel_strong_3D_1.wlz
3268_voxel_notDetected_3D_2.wlz
3268_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3268_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
notochord
detected detected
Expression is detected in the notochord adjacent to the hindbrain.
notochordal plate
detected detected
Expression is detected in the prechordal plate adjacent to the forebrain.
hindgut diverticulum endoderm
detected detected
Expression is detected in hindgut endoderm.
primitive streak
detected detected
Expression is detected in the node.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2158747
Entity Detected:Shh, sonic hedgehog ( MGI:98297)
Notes:The Shh probe used in this study by Lowe et al., 2001 [PMID:11311163] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:129/Sv * C57BL/6J
Age:8.5 dpc
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Lowe LA; Yamada S; Kuehn MR. 2001 [PMID:11311163] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11311163] Lowe LA, Yamada S, Kuehn MR 2001 Genetic dissection of nodal function in patterning the mouse embryo. Development (128):1831-43
Links:MGI:2159422 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI