EMAGE mouse gene expression spatial database

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EMAGE:3275

Smad3 MAD homolog 3 (Drosophila) ( MGI:1201674)
TS14 (9.5 dpc)
in situ hybridisation

Data Images


Fig 7B, Tremblay et al, 2000 [PMID:10862745] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.	Fig 7D, Tremblay et al, 2000 [PMID:10862745] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.	Fig 7F, Tremblay et al, 2000 [PMID:10862745] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Image annotations: n - notochord, fg, foregut, hg - hindgut, mg - midgut. The three data images below are from one serially sectioned embryo. Note that only the data in Images B and D have been spatially mapped in this EMAGE entry. Image annotations: hg - hindgut; fg - foregut; n - notochord; mg - midgut.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3275_voxel_possible_3D_1.wlz

3275_voxel_strong_3D_1.wlz

3275_voxel_moderate_3D_1.wlz

3275_voxel_possible_3D_2.wlz

3275_voxel_strong_3D_2.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3275_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

detected

homogeneous

foregut

strong

homogeneous

hindgut diverticulum

strong

homogeneous

midgut

strong

homogeneous

extraembryonic component

not detected

homogeneous

There is no expression in the visceral yolk sac endoderm

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1860522
Entity Detected:	Smad3, MAD homolog 3 (Drosophila) ( MGI:1201674)
Sequence:	sense strand is shown >MGI:1860522 GGATCTCGGTGTGGCGTGGCACCAACACTGGAGGTAGAACTGGCGTCTCTACTCTCTGATAGTGGTAAGG ATTTACACACACTTCATCCTTCTTCATGTTGAAGGCAAACTCACAGAGCTCCATGGCCCGTAATTCATGG TGGCTGTGCAGGTCGGGCCATCGCCACAGGCTGCAGTAGATAACGTGAGGGAGCCCCTTCCGATGGGACA CCTGCAGCCGACCATCCAGTGACCTGGGGATGGTAATGCACTTGGTGTTCACGTTCTGCGTGGTGATGGC CTTCTCCAGCTCGTCCAACTGCCCCGTCTTCTTGAGCTTCTTCACCAAGCTCTTGACCGCCTTCTCGCAC CACTTCTCCTCCTGCCCGTTCTGCTCGCCCTTCTTCCAACCCAGCAGGCGCTTCACGATCGGGGGGGTGA AGGGCAGGATGGACGACATGGCTGGCACCGAAGG
Notes:	The Smad3 probe used in this study by Tremblay et al, 2000 [PMID:10862745] was described as "a 424 bp Smad3 EST corresponding mostly to 5'UTR (GenBank accession no. AA023641) ".
Chemistry:	RNA
Strand:	antisense
Label:	unspecified radioactivity

Specimen

Organism:	mouse
Age:	9.5 dpc
Theiler Stage:	TS14
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	4% paraformaldehyde
Embedding:	paraffin
Staining procedure:	autoradiography

General Information

Authors:	Tremblay KD; Hoodless PA; Bikoff EK; Robertson EJ 2000 [PMID:10862745] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:10862745] Tremblay KD, Hoodless PA, Bikoff EK, Robertson EJ 2000 Formation of the definitive endoderm in mouse is a Smad2-dependent process. Development (127):3079-90
Links:	MGI:1860609 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source