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EMAGE:3278

Msx1 homeobox, msh-like 1 ( MGI:97168)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:3278 EMAGE:3278 EMAGE:3278 EMAGE:3278 EMAGE:3278
Fig5A (darkfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5A (brightfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5B (brightfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5B (darkfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5C (brightfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.
EMAGE:3278 EMAGE:3278 EMAGE:3278 EMAGE:3278 EMAGE:3278
Fig5C (darkfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5D (brightfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5D (darkfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5E (brightfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5E (darkfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.
EMAGE:3278 EMAGE:3278
Fig5F (brightfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig5F (darkfield) Mackenzie et al, 1991 [PMID:1680043] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: Coronal sections. Scale bar = 50 microns. A - F are rostral to caudal series of sections. Image Annotations: M, medial process; L, lateral proces; C, mesenchymal capsule; B, brain; MN, meninges; N, primary nares; MD, mandibular process; MX, maxillary process; T, tongue anlage; 2, second branchial arch. Arrows in D and E point to thickened dental epithelia.
Expression Pattern Description
Spatial Annotation:
EMAGE:3278EMAGE:3278EMAGE:3278EMAGE:3278EMAGE:3278Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mapping3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3278_voxel_notDetected_3D_1.wlz
3278_voxel_strong_3D_1.wlz
3278_voxel_moderate_3D_1.wlz
3278_voxel_notDetected_3D_2.wlz
3278_voxel_strong_3D_2.wlz
3278_voxel_notDetected_3D_3.wlz
3278_voxel_strong_3D_3.wlz
3278_voxel_notDetected_3D_4.wlz
3278_voxel_strong_3D_4.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3278_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
skeleton
detected detected
regionalLabeling in the future skull bones (Figs 5abcdef)
brain
detected detected
regionalExpression in the brain and in meninges (Fig 5abcdef)
future adenohypophysis
detected detected
regionalExpression in the anterior pituitary
latero-nasal process
detected detected
regionalIn the region of closure between the medial nasal, lateral nasal, and maxillary processes there is expression throughout the mesenchyme and in the epithelia of the oral stem of the Y-shaped nares (Fig 5b).
medial-nasal process
detected detected
regionalIn the region of closure between the medial nasal, lateral nasal, and maxillary processes there is expression throughout the mesenchyme and in the epithelia of the oral stem of the Y-shaped nares (Fig 5b).
maxillary process
detected detected
regionalIn the region of closure between the medial nasal, lateral nasal, and maxillary processes there is expression throughout the mesenchyme and in the epithelia of the oral stem of the Y-shaped nares (Fig 5b). Expression in the mesenchyme surrounding the thickened epithelium that fell off progressively in an anterioposterior gradient (Fig 5cde).
mandibular process mesenchyme
detected detected
regionalLabeling in the mesenchyme at the tip of the mandibular arch (Fig 5c). In the anterior and middle thirds of the mandibular arch expression decreased progressively in the caudal, aboral mesenchyme and in more posterior arch mesenchyme (Fig 5de).
oral region epithelium
not detected not detected
homogeneousNo expression in the mandibular epithelium (Fig 5cdef).
tongue
detected detected
regionalLabelling was intense in the anterior two thirds of the tongue anlage (Fig 5d) but absent in the posterior third (Fig 6).
2nd branchial arch mesenchyme
detected detected
Fig 5f
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:12837
Entity Detected:Msx1, homeobox, msh-like 1 ( MGI:97168)
Notes:The probe used in this study by Mackenzie et al , 1991 [PMID:1680043] is depicted in Fig 1 of that paper and described as a "600bp EcoRI fragment", "containing 375bp of coding sequence". Exact end positions are unknown as these values are ambiguous when comparing to other Msx1 cDNA sequences (eg. NM_010835.1)
Chemistry:RNA
Strand:antisense
Label:unspecified radioactivity
Specimen
Organism:mouse
Strain:Manchester
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Mackenzie et al , 1991 [PMID:1680043] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1680043] Mackenzie A, Leeming GL, Jowett AK, Ferguson MW, Sharpe PT 1991 The homeobox gene Hox 7.1 has specific regional and temporal expression patterns during early murine craniofacial embryogenesis, especially tooth development in vivo and in vitro. Development (0):269-85
Links:MGI:2136379 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI