Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3283

Nkx2-5 NK2 transcription factor related, locus 5 (Drosophila) ( MGI:97350)
TS11 (7.5 dpc)
in situ hybridisation

Data Images
EMAGE:3283 EMAGE:3283 EMAGE:3283 EMAGE:3283 EMAGE:3283
Fig4A (top, darkfield) Clark et al, 1999 [PMID:10331975] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig4 Clark et al, 1999 [PMID:10331975] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig4A (bottom, brightfield) Clark et al, 1999 [PMID:10331975] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig4B (bottom, brightfield) Clark et al, 1999 [PMID:10331975] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright. Fig4B (top, darkfield) Clark et al, 1999 [PMID:10331975] .Copyright: This image is from Development and is displayed with the permission of the Company of Biologists Ltd who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Approximate section planes are shown on the cartoon image. Image annotations: asterisk = anterior. Corresponding pairs of dark- and bright-field images are shown. Positions of the section planes are shown in the cartoon. Specimen also refered to as late allantoic bud stage. Image annotations: * = anterior.
Expression Pattern Description
Spatial Annotation:
EMAGE:3283EMAGE:3283EMAGE:3283Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mapping3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3283_voxel_notDetected_3D_1.wlz
3283_voxel_strong_3D_1.wlz
3283_voxel_notDetected_3D_2.wlz
3283_voxel_strong_3D_2.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3283_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
cardiogenic plate
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1303179
Entity Detected:Nkx2-5, NK2 transcription factor related, locus 5 (Drosophila) ( MGI:97350)
Sequence:sense strand is shown

>MGI:1303179
GATCTGGTTCCAGAACCGTCGCTACAAGTGCAAGCGACAGCGGCAGGACCAGACTCTGGAGCTTCTGGGG
CCGCCGCCGCCGCCCGCGCGCAGGATCGCGGTGCCCGTGCTGGTGCGCGACGGGAAGCCCTGCCTGGGGG
ACCCCGCGGCCTACGCTCCCGCCTACGGCGTGGGTCTCAATGCCTATGGCTACAACGCCTACCCCTACCC
CAGCTACGGCGGCGCGGCCTGCAGTCCCGGCTACAGCTGCGCCGCCTACCCCGCTGCGCCCCCCGCCGCG
CAGCCCCCCGCCGCCTCCGCCAACAGCAACTTCGTGAACTTTGGCGTCGGGGACTTGAACACCGTGCAGA
GTCCCGGGATGCCGCAGGGCAATTCGGGCGTCTCCACGCTGCACGGCATCCGAGCCTGGTAGGGAAAGAG
CCCGTTTGGGGCGCCCCGGAACGACTCCCACCTTTAGGAGAAGGGCGATGACTCCGGGGATGGGAAAGCT
CCCACTATGCCCTGTCCCTCGGATTTCACACCCACCCTCGCGCAGGCCTGGGACCTTTCTCCGATCCATC
CCACTTTATTGACGTAGCCTGGTGTCTCGGACCTGGCAGAGCCTTCCAGGGTCACGGGCACTTTCGACGG
ATTCCACACTAGGACCGGGAGCCTGGGCCGGGCGCCCGGGCCCTGGTTGTCTTGCCGTCGCCCACCCACC
CGTATTTATGTTTTTACCTGTTGTAAGAAATGAGAACCCTCTTCCCATTAAAGTGAGTGCGTAACGCAAG
GAATT
nt 821 - nt 1595 of X75415.1
Notes:The probe used in this study by Clark et al, 1999 [PMID:10331975] is defined as "corresponding to nucleotides 821- 1595 of the published sequence (Lints et al, 1993 [PMID:7904557] )". GenBank sequence X75415 corresponds to this sequence.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Age:7.5 dpc
Theiler Stage:TS11
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Clark et al, 1999 [PMID:10331975] Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10331975] Clark TG, Conway SJ, Scott IC, Labosky PA, Winnier G, Bundy J, Hogan BL, Greenspan DS 1999 The mammalian Tolloid-like 1 gene, Tll1, is necessary for normal septation and positioning of the heart. Development (126):2631-42
 [ PMID:7904557] Lints TJ, Parsons LM, Hartley L, Lyons I, Harvey RP 1993 Nkx-2.5: a novel murine homeobox gene expressed in early heart progenitor cells and their myogenic descendants. Development (119):419-31
Links:MGI:2450519 same experiment
 EMAGE:3252 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI