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EMAGE:3292

Col2a1 collagen, type II, alpha 1 ( MGI:88452)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3292
Figure 5B of Cheah et al., 1991 [PMID:1879363] . Copyright: This image is from Cheah et al., Development 111:945-53 (1991) and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Abbreviations: v, ventricle; at, atrium; ba, branchial arch; rp, hypophyseal pouch.
Expression Pattern Description
Spatial Annotation:
EMAGE:3292EMAGE:3292Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3292_voxel_strong_3D_1.wlz
3292_voxel_moderate_3D_1.wlz
3292_voxel_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3292_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
common atrial chamber right part cardiac muscle
detected detected
common atrial chamber left part cardiac muscle
detected detected
primitive ventricle cardiac muscle
detected detected
common atrial chamber left part endocardial lining
not detected not detected
homogeneous
common atrial chamber right part endocardial lining
not detected not detected
homogeneous
primitive ventricle endocardial lining
not detected not detected
homogeneous
left auricular region endocardial lining
not detected not detected
homogeneous
left auricular region cardiac muscle
detected detected
right auricular region endocardial lining
not detected not detected
homogeneous
right auricular region cardiac muscle
detected detected
pericardial component
not detected not detected
homogeneousExpression was absent from the pericardium.
heart
detected detected
Expression is detected in the epicardium
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23585
Entity Detected:Col2a1, collagen, type II, alpha 1 ( MGI:88452)
Notes:The probe used in this study by Cheah et al., 1991 [PMID:1879363] is either "an 850 bp PstI-SalI DNA fragment which hybridised strongly to the 5' first exon of the human gene" (pEL107) or "a shorter derivative [of pEL101], pEL111, containing the 600 bp AccI-SalI DNA fragment" (see Figure 1 therein). Editor's note: it is unclear which of these probes was used for this assay, however the authors report that both probes yield 'similar results'.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:CBA/N
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Cheah KS; Lau ET; Au PK; Tam PP, 1991 [PMID:1879363] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1879363] Cheah KS, Lau ET, Au PK, Tam PP 1991 Expression of the mouse alpha 1(II) collagen gene is not restricted to cartilage during development. Development (111):945-53
Links:MGI:3042268 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI