EMAGE mouse gene expression spatial database

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EMAGE:3307

Fst follistatin ( MGI:95586)
TS20 (12.5 dpc)
in situ hybridisation

Data Images


Figure 9E of Feijen et al., 1994 [PMID:7821227] . Copyright: This image is from Feijen et al., Development 120, 3621-3637 (1994)), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.	Figure 9E of Feijen et al., 1994 [PMID:7821227] . Copyright: This image is from Feijen et al., Development 120, 3621-3637 (1994)), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
Find spatially similar expression patterns:

Notes:

Note: This is a sagittal section through abdominal region. fa, fascia; lb, limb bud; li, liver; pe, pelvis; st, stomach.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

3D mapping

spatial mapping

View mapped 3D expression image

Download individual expression domains:

3307_voxel_strong_3D_1.wlz

3307_voxel_moderate_3D_1.wlz

3307_voxel_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3307_all_domains.zip

Find spatially similar expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

stomach mesenchyme

strong

regional

Underlying mesenchymal tissue, but not in the muscle tissue.

trunk mesenchyme

detected

regional

Fascia in the flanks.

limb

detected

regional

Mesenchymal condensations.

skeleton

detected

regional

Mesenchymal condensations in the developing pelvis.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:23936
Entity Detected:	Fst, follistatin ( MGI:95586)
Sequence:	sense strand is shown >MGI:23936 GCTTCCTCTGAGCAATATCTCTGTGGGAATGATGGAGTCACCTACTCCAGTGCCTGCCACCTGAGAAAGG CTACCTGCCTGCTGGGCAGATCTATTGGATTAGCCTATGAGGGAAAGTGTATCAAAGCAAAGTCCTGTGA AGATATCCAGTGCACTGGTGGGAAAAAATGTTTATGGGATTTCAAGGTTGGGAGAGGCCGGTGTTCCCTC TGTGATGAGCTGTGCCCTGACAGTAAGTCGGATGAGCCTGTCTGTGCCAGTGACAATGCCACTTATGCCA GCGAGTGTGCCATGAAGGAAGCTGCCTGCTCCTCAGGTGTGCTACTG
	nt 625 - nt 951 of NM_006350.2
Notes:	The Fst probe used in this study by Feijen et al., 1994 [PMID:7821227] was described as "a 324 nucleotide fragment, spanning amino acid residues 171-279 (Shimasaki et al., 1988 [PMID:3380788] )". Editor's note: The co-ordinates delineated above against NM_006350.2 were deduced by comparing the sequence shown in Fig2 of Shimasaki et al., 1988 and those in GenBank.
Chemistry:	RNA
Strand:	antisense
Label:	S35

Specimen

Organism:	mouse
Strain:	(C57BL/6 x CBA)F1
Age:	12.5 dpc
Theiler Stage:	TS20
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	4% paraformaldehyde
Embedding:	paraffin
Staining procedure:	autoradiography

General Information

Authors:	Feijen A; Goumans MJ; van den Eijnden-van Raaij AJ, 1994 [PMID:7821227] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:7821227] Feijen A, Goumans MJ, van den Eijnden-van Raaij AJ 1994 Expression of activin subunits, activin receptors and follistatin in postimplantation mouse embryos suggests specific developmental functions for different activins. Development (120):3621-37
	[ doi:10.1073/pnas.85.12.4218] [ PMID:3380788] Shimasaki S, Koga M, Esch F, Cooksey K, Mercado M, Koba A, Ueno N, Ying SY, Ling N, Guillemin R 1988 Primary structure of the human follistatin precursor and its genomic organization. Proc Natl Acad Sci U S A (85):4218-22
Links:	MGI:2135921 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source