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EMAGE:3312

Gata3 GATA binding protein 3 ( MGI:95663)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3312 EMAGE:3312
Figure 4I of Ruest et al., 2004 [PMID:15306564] . Copyright: This image is from Ruest LB, Development 2004 Sep;131(18):4413-23 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 4K of Ruest et al., 2004 [PMID:15306564] . Copyright: This image is from Ruest LB, Development 2004 Sep;131(18):4413-23 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: 1= mandibular region of the first pharyngeal arch; 2= second pharyngeal arch; h= heart; lb= limb bud; np= nasal prominence.
Expression Pattern Description
Spatial Annotation:
EMAGE:3312Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3312_wholemount_strong_3D_1.wlz
3312_wholemount_moderate_3D_1.wlz
3312_wholemount_weak_3D_1.wlz
3312_wholemount_possible_3D_1.wlz
3312_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3312_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component mesenchyme
detected detected
regionalExpression is distal.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:23164
Entity Detected:Gata3, GATA binding protein 3 ( MGI:95663)
Sequence:sense strand is shown

>MGI:23164
GCTAAACTATCCCGCAAAGATTTTTCTTTCCTCCCTAAACCCTCCTTTTTGCTCTCCTTTTCTATACCCT
TAACTGCAAACAAACCATTAAACGACCCCTCTCCTGGGCCTCCGACGGCAGGAGTCCGCGGACCTCCCAG
GCCGACAGCCCTCCCTCTACCCGCGAGGTTCCGGGCCGGGCGAGAGGGCGCGAGCACAGCCGAGGACATG
GAGGTGACTGCGGACCAGCCGCGCTGGGTGAGCCACCATCACCCCGCGGTCCTCAACGGTCAGCACCCAG
ACACGCACCACCCGGGCCTCGGCCATTCGTACATGGAAGCTCAGTATCCGCTGACGGAAGAGGTGGACGT
ACTTTTTAACATCGATGGTCAAGGCAACCACGTCCCGTCCTACTACGGAAACTCCGTCAGGGCTACGGTG
CAGAGGTATCCTCCGACCCACCACGGGAGCCAGGTATGCCGCCCGCCTCTGCTGCACGGATCTCTGCCCT
GGCTGGATGGCGGCAAAGCCCTGAGCAGCCACCACACCGCCTCGCCCTGGAACCTCAGCCCCTTCTCCAA
GACGTCCATCCACCACGGCTCTCCGGGGCCTCTGTCCGTTTACCCTCCGGCTTCATCCTCTTCTCTGGCG
GCCGGCCACTCCAGTCCTCATCTCTTCACCTTCCCGCCCACCCCGCCGAAAGACGTCTCCCCAGACCCGT
CGCTGTCCACCCCGGGATCCGCCGGGTCGGCCAGGCAAGATGAGAAAGAGTGCCTCAAGTATCAGGTGCA
GCTGCCAGATAGCATGAAGCTGGAGACGTCTCACTCTCGAGGCAGCATGACCACCCTGGGTGGGGCCTCA
TCCTCAGCCCACCACCCCATTACCACCTATCCGCCCTATGTGCCCGAGTACAGCTCTGGACTCTTCCCAC
CCAGCAGCCTGCTGGGAGGATCCCCTACCGGGTTCGGATGTAAGTCGAGGCCCAAGGCACGATCCAGCAC
AGAAGGCAGGGAGTGTGTGAACTGCGGGGCAACCTCTACCCCACTGTGGCGGCGAGATGGTACCGGGCAC
TACCTTTGCAATGCCTGCGGACTCTACCATAAAATGAATGGGCAGAACCGGCCCCTTATCAAGCCCAAGC
GAAGGCTGTCGGCAGCAAGGAGAGCAGGGACATCCTGCGCGAACTGTCAGACCACCACCACCACCCTCTG
GAGGAGGAACGCTAATGGGGACCCGGTCTGCAATGCCTGTGGGCTGTACTACAAGCTTCATAATATTAAC
AGACCCCTGACTATGAAGAAAGAAGGCATCCAGACCCGAAACCGGAAGATGTCTAGCAAATCGAAAAAGT
GCAAAAAGGTGCATGACGCGCTGGAGGACTTCCCCAAGAGCAGCTCCTTCAACCCGGCCGCTCTCTCCAG
ACACATGTCATCCCTGAGCCACATCTCTCCCTTCAGCCACTCCAGCCACATGCTGACCACACCGACGCCC
ATGCATCCGCCCTCCGGCCTCTCCTTCGGACCTCACCACCCTTCCAGCATGGTCACCGCCATGGGTTAGA
GAGGCAGAGCCCTGCTCCACATGCGTGAGGAGTCTCCAAGTGTGCGAAGAGTTCCTCCGACCCCTTCTAC
TTGCGTTTTTCGCAGGAGCAGTATCATGAAGCCCGAAAGCGACAGATCTGTGTTTTTGAAGGCAGAAAGC
AAAATGTTTGCTTCTTTTTTCAAAGGAGCTCGAGGTGGTGTCTGCATTCCAACCACTGAATCCGGATCCC
ATTTGTGAATAAGCCATTCAGACTCATATTCCCTATTTAACAGGGTCTCTAGTGCTGTGAAAAAAATATT
GCTGAACATTGCATATAACTTATATTGTAAGAAATACTGTACATTTGAGGAAGACTTTATTGTACCTGGA
TAGCTGTAAGAAAGGCATGAAGGACGCCAAGAGTTTTAAGGAATATAGGGGNNTTAAAGTATGGAGATAC
AGAAGAAACCACTAAGTCTGATGTCCAAATGGGCACACTGTCAGTTTTGTTTCCCTTCAGTTGTTTGATG
CATTTAAAAAAAAAAAAAAGAAAGAA
nt 1 - nt 2056 of X55123.1
Notes:The Gata3 probe used in this study by Ruest et al., 2004 [PMID:15306564] is indicated as that originally described by George et al., 1994 [PMID:7956841] i.e. a "linearized exoIII-deletion of the full-length cDNA, mc5b1 (Ko et al., 1991 [PMID:2017177] )". See Figure 2 in Ko et al., 1991 [PMID:2017177] and accompanying GenBank entry X55123.1 for the insert sequence of clone mc5b.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ruest LB; Xiang X; Lim KC; Levi G; Clouthier DE, 2004 [PMID:15306564] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01291] [ PMID:15306564] Ruest LB, Xiang X, Lim KC, Levi G, Clouthier DE 2004 Endothelin-A receptor-dependent and -independent signaling pathways in establishing mandibular identity. Development (131):4413-23
 [ PMID:7956841] George KM, Leonard MW, Roth ME, Lieuw KH, Kioussis D, Grosveld F, Engel JD 1994 Embryonic expression and cloning of the murine GATA-3 gene. Development (120):2673-86
 [ PMID:2017177] Ko LJ, Yamamoto M, Leonard MW, George KM, Ting P, Engel JD 1991 Murine and human T-lymphocyte GATA-3 factors mediate transcription through a cis-regulatory element within the human T-cell receptor delta gene enhancer. Mol Cell Biol (11):2778-84
Links:MGI:3510696 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI