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EMAGE:3317

Gbx2 gastrulation brain homeobox 2 ( MGI:95668)
TS12 (7 Somite no.)
in situ hybridisation

Data Images
EMAGE:3317
Figure 7J of Liu et al., 1999 [PMID:10518499] .Copyright: This image is from Liu A, Development 1999;126():4827-38, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Green arrowhead points to the anterior neural plate. Red arrowhead points to the mid/hindbrain junction as defined by the caudal border of Wnt1 and Otx2 expression, and the rostral border of Fgf8 and Gbx2 expression.
Expression Pattern Description
Spatial Annotation:
EMAGE:3317Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3317_wholemount_strong_3D_1.wlz
3317_wholemount_moderate_3D_1.wlz
3317_wholemount_possible_3D_1.wlz
3317_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3317_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future hindbrain
detected detected
The midbrain/hindbrain border is marked by the rostral limit of expression.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:26910
Entity Detected:Gbx2, gastrulation brain homeobox 2 ( MGI:95668)
Sequence:sense strand is shown

>MGI:26910
GGCGCCCGCCAGAGACAAGTGCGCCGGCTTCGCGCGCTCCCCAGCCGCCCGTGGGAGACGACGGACTGCA
GGACGGACAGACGGACGGACGCACGCACTGCTGGTAGCTGGGCCGAGACCTGCAGACGCGAAGCGCGCGC
GTTCCGACGGGCTAGCAGCCGGGAGCGGTTCCGGCGAACGCGAGGTCCCCGGCGCCCTCTCCATCCCGGG
CGCACGGCCTCATCGCGCTCGGTGGCTGCACCCGTGCAGGCCGGGTATGCTGCGGGTCCTGCACTGAGGG
GCGGTGGTGGTGTCTGTGCTCTGGGCACCGCTCAGCCCCTGCCCGGAGTTCCCGGTGGTACTCCCAGCGT
CCGGAGGGCTTCCAGGCGGCGGCGGGACACGGGGACTTTTCGTCTCTCCCTGGCTTCTCCCGAACGCGTC
TATGAGCGCAGCGTTCCCGCCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGTACCGCCTTCAGC
ATAGACTCGCTGATCGGCAGCCCGCCGCAGCCCAGTCCCGGCCATTTCGTCTACACCGGCTACCCCATGT
TCATGCCCTACCGGCCGGTGGTGCTGCCGCCACCGCCGCCACCGCCTCCCGCGCTGCCCCAGGCAGCGCT
GCAGCCCGCTCTGCCGCCCGCGCACCCTCACCACCAGATCCCCAGCCTGCCCACCGGCTTCTGCTCCAGC
CTGGCGCAGGGCATGGCGCTCACCTCCACGCTCATGGCCACTCTGCCCGGCGGCTTCTCTGCGTCGCCCC
AGCACCAAGAGGCGGCGGCTGCCCGCAAGTTCGCTCCACAGCCACTGCCCGGAGGCGGCAACTTCGACAA
AGCCGAGGCGCTCCAAGCGGATGCGGAAGACGGCAAAGCCTTCTTGGCCAAGGAGGGCTCGCTGCTCGCT
TTCTCTGCGGCCGAAGCGGTGCAGGCGTCGCTCGTCGGGGCTGTCCGAGGGCAAGGGAAAGACGAGTCAA
AGGTGGAAGATGACCCGAAGGGCAAGGAGGAGAGCTTCTCTCTGGAGAGCGATGTGGATTACAGCTCAGA
TGACAATTTGCCTGGTCAGACTGCTCATAAGGAAGAAGACCCCGGCCACGCACTGGAGGAGACCCCGCAG
AGCGGCGGTGCAGCAGGCAGCACCACGTCCACAGGCAAGAACCGGCGGCGGCGGACTGCCTTCACCAGCG
AACAGCTGCTGGAGCTGGAGAAAGAATTCCACTGCAAAAAGTACCTCTCCCTGACCGAGCGCTCACAGAT
CGCCCACGCCCTCAAACTCAGCGAGGTGCAAGTAAAAATCTGGTTCCAGAACCGCCGGGCCAAGTGGAAA
CGTGTCAAGGCAGGCAACGCCAATTCCAAGACGGGGGAGCCCTCTCGGAACCCCAAGATTGTCGTCCCCA
TCCCTGTTCACGTTAGCAGGTTCGCTATTCGAAGTCAACACCAGCAGCTGGAGCAGGCCCGACCCTGAGG
GCCCAGCCAGGGTGGACACCACCATGGAGAGGCCCTAACACCTGAAGGAACCCGTGTCAGACTCCACTGT
TTAAAGCAGAACTCAGAGGCACCTCCTAGATGTGGACATCCAAGCAAATTGAGAGTATATTCATTAGACG
GGCTTAAAGGGGGCTACAGCCACACCAGAAGATACACTAAATATTTATTATACAACCCTACTTTGTACAT
AGATCTCTATCTAGACTGGATCTCAGCTGCAGTGTTTTGAAAGGGATAGGATCAAATCTCTCTCCTTACA
CTTTCCAGATGAAACAAAACCACCACTGTACTCTGTCGCCTTCAGCGACCACCTTCCCATACGTGCCCAA
AGGTAAACAGGAGACACAAGGACAAACACTGCGGTTTAACCTTCTTATTGTTATTTTTTGTTTGTTTGTT
TCTATTTTGGCTTTTTGAGTTGAGTGCAGAGCAGTCGGTTGATTTTGGAGCAATCTAGAACTCTGCTCTG
CTCGTTTGCTTTGCCTGGCTGTCTTTCCTGGCCTTGTATATCCTATCTGAGCCATGTCGGAAGCACGTCT
CCGTTGTGTTTAATTTATTTCAATGTAGCTTATTTTCATATAAGTTATGACATTTAAACAATCTCAGTCT
TGTGAATAATAAAAAAAAAGGCGAGG
nt 1 - nt 2126 of Z48800.1
Notes:The probe used in this study by Liu et al., 1999 [PMID:10518499] is indicated as that described by Bouillet et al., 1995 [PMID:8601031] i.e. a "2.1 kb Stra7/Gbx-2 cDNA". Editor's note: The 2.1kb sequence is depicted in Fig 1 therein and was submitted to GenBank by P. Bouillet (and subsequently assigned the ID Z48800).
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:7 Somite no.
Theiler Stage:TS12
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Liu A; Losos K; Joyner AL, 1999 [PMID:10518499] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10518499] Liu A, Losos K, Joyner AL 1999 FGF8 can activate Gbx2 and transform regions of the rostral mouse brain into a hindbrain fate. Development (126):4827-38
 [ doi:10.1002/aja.1002040404] [ PMID:8601031] Bouillet P, Chazaud C, Oulad-Abdelghani M, Dolle P, Chambon P 1995 Sequence and expression pattern of the Stra7 (Gbx-2) homeobox-containing gene induced by retinoic acid in P19 embryonal carcinoma cells. Dev Dyn (204):372-82
Links:MGI:1349181 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI