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EMAGE:3320

Gbx2 gastrulation brain homeobox 2 ( MGI:95668)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3320
Figure 8G of Boyl et al., 2001 [PMID:11532921] .Copyright: This image is from Boyl PP, Development 2001;128():2989-3000, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3320EMAGE:3320Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3320_voxel_strong_3D_1.wlz
3320_voxel_moderate_3D_1.wlz
3320_voxel_possible_3D_1.wlz
3320_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3320_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
midbrain
detected detected
regionalExpression is at the midbrain/hindbrain boundary.
hindbrain
detected detected
regionalExpression is at the midbrain/hindbrain boundary and in the future isthmus.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1321259
Entity Detected:Gbx2, gastrulation brain homeobox 2 ( MGI:95668)
Sequence:sense strand is shown

>MGI:1321259
CCGTCGCTGATGATGATGCAGCGCCCGCTGGGGAGTAGTACCGCCTTCAGCATAGACTCGCTGATCGGCA
GCCCGCCGCAGCCCAGTCCCGGCCATTTCGTCTACACCGGCTACCCCATGTTCATGCCCTACCGGCCGGT
GGTGCTGCCGCCACCGCCGCCACCGCCTCCCGCGCTGCCCCAGGCAGCGCTGCAGCCCGCTCTGCCGCCC
GCGCACCCTCACCACCAGATCCCCAGCCTGCCCACCGGCTTCTGCTCCAGCCTGGCGCAGGGCATGGCGC
TCACCTCCACGCTCATGGCCACTCTGCCCGGCGGCTTCTCTGCGTCGCCCCAGCACCAAGAGGCGGCGGC
TGCCCGCAAGTTCGCTCCACAGCCACTGCCCGGAGGCGGCAACTTCGACAAAGCCGAGGCGCTCCAAGCG
GATGCGGAAGACGGCAAAGCCTTCTTGGCCAAGGAGGGCTCGCTGCTCGCTTTCTCTGCGGCCGAAGCGG
TGCAGGCGTCGCTCGTCGGGGCTGTCCGAGGGCAAGGGAAAGACGAGTCAAAGGTGGAAGATGACCCGAA
GGGCAAGGAGGAGAGCTTCTCTCTGGAGAGCGATGTGGATTACAGCTCAGATGACAATTTGCCTGGTCAG
ACTGCTCATAAGGAAGAAGACCCCGGCCACGCACTGGAGGAGACCCCGCAGAGCGGCGGTGCAGCAGGCA
GCACCACGTCCACAGGCAAGAACCGGCGGCGGCGGACTGCCTTCACCAGCGAACAGCTGCTGGAGCTGGA
GAAAGAATTCCACTGCAAAAAGTACCTCTCCCTGACCGAGCGCTCACAGATCGCCCACGCCCTCAAACTC
AGCGAGGTGCAAGTAAAAATCTGG
nt 441 - nt 1304 of NM_010262.1
Notes:The probe used in this study by Boyl et al., 2001 [PMID:11532921] is indicated as that originally described in Acampora et al., 1998 [PMID:9811592] as a "PCR fragment including the region between aa 6 and aa 294". Editor's note: the co-ordinates shown above were deduced from this information.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Staining procedure:autoradiography
General Information
Authors:Boyl PP; Signore M; Acampora D; Martinez-Barbera JP; Ilengo C; Annino A; Corte G; Simeone A, 2001 [PMID:11532921] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:11532921] Boyl PP, Signore M, Acampora D, Martinez-Barbera JP, Ilengo C, Annino A, Corte G, Simeone A 2001 Forebrain and midbrain development requires epiblast-restricted Otx2 translational control mediated by its 3' UTR. Development (128):2989-3000
 [ PMID:9811592] Acampora D, Avantaggiato V, Tuorto F, Briata P, Corte G, Simeone A 1998 Visceral endoderm-restricted translation of Otx1 mediates recovery of Otx2 requirements for specification of anterior neural plate and normal gastrulation. Development (125):5091-104
Links:MGI:2150155 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI