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EMAGE:3325

Gja1 gap junction protein, alpha 1 ( MGI:95713)
TS07 (5.5 dpc)
immunohistochemistry

Data Images
EMAGE:3325
Figure 1A of Yancey et al., 1992 [PMID:1315676] .Copyright: This image is from Yancey SB, Development 1992 Jan;114(1):203-12, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: Arrows point to punctate staining. ec= primitive ectoderm, en= primitive endoderm, d= decidua, arrows= embryonic expression. Bar= 100um.
Expression Pattern Description
Spatial Annotation:
EMAGE:3325EMAGE:3325Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3325_voxel_strong_3D_1.wlz
3325_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3325_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
epiblast
weak weak
spottedExpression is primarily in the primitive endoderm.
visceral extraembryonic endoderm
weak weak
spottedExpression is primarily in the primitive endoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1338490
Entity Detected:Gja1, gap junction protein, alpha 1 ( MGI:95713)
Antigen:sense strand is shown

>MGI:1338490
DQRPSSRASSRASSRPRPDDLEI
aa 360 - aa 382 of NP_034418.1
Notes:The anti Gja1 (Cx43/connexin 43) antisera used in this study by Yancey et al., 1992 [PMID:1315676] was "raised against a synthetic peptide representing amino acids 360-382 located at the carboxy terminus of the Cx43. It is highly specific for Cx43 (Laird & Revel, 1990 [PMID:2175311] : Yancey, unpublished)."
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Strain:BALB/c
Age:5.5 dpc
Theiler Stage:TS07
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:paraffin
Visualisation method:fluorescence
General Information
Authors:Yancey SB; Biswal S; Revel JP, 1992 [PMID:1315676] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:1315676] Yancey SB, Biswal S, Revel JP 1992 Spatial and temporal patterns of distribution of the gap junction protein connexin43 during mouse gastrulation and organogenesis. Development (114):203-12
 [ PMID:2175311] Laird DW, Revel JP. 1990 Biochemical and immunochemical analysis of the arrangement of connexin43 in rat heart gap junction membranes. J Cell Sci (97 ( Pt 1)):109-17
Links:MGI:1338492 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI