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EMAGE:3333

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS15 (9.2 dpc)
in situ hybridisation

Data Images
EMAGE:3333
Fig 2C of Mallo, 1997 [PMID:9142993] . Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1997.8519] Dev Biol 184: 175-86, Mallo M, Retinoic acid disturbs mouse middle ear development in a stage-dependent fashion. Copyright 1997

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3333Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3333_wholemount_strong_3D_1.wlz
3333_wholemount_moderate_3D_1.wlz
3333_wholemount_weak_3D_1.wlz
3333_wholemount_possible_3D_1.wlz
3333_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3333_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
2nd branchial arch mesenchyme derived from neural crest
detected detected
head mesenchyme derived from neural crest
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:14729
Entity Detected:Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Sequence:sense strand is shown

>MGI:14729
CCAGCGTGCGACCGCTCCGCAGCAGAGGTGGGTGCCTGCCCCTGCCTTCGCGCCGCCGTACAGCCAACAC
CACTGCCACCATGCCCAACTTCGCCGGTACCTGGAAGATGCGCAGCAGCGAGAATTTCGACGAGCTCCTC
AAGGCGTTGGGTGTGAACGCCATGCTGAGGAAGGTGGCCGTGGCGGCTGCGTCTAAGCCGCACGTGGAGA
TCCGCCAAGACGGGGATCAGTTCTACATCAAGACATCCACTACTGTGCGCACCACGGAGATCAACTTCAA
GGTCGGAGAGGGCTTCGAGGAGGAGACAGTGGACGGACGCAAATGCAGGAGTTTACCCACGTGGGAGAAT
GAGAACAAGATTCACTGCACACAGACTCTTCTGGAGGGGGATGGCCCCAAAACTTACTGGACCCGAGAGC
TGGCCAACGATGAGCTCATCCTGACATTTGGCGCCGATGATGTGGTCTGCACGAGGATTTATGTCCGGGA
GTGAAAGGTGGCCAGCTTGTTCCTGCTTCATGACGATGCGAGTTCCCCTGAGGGGTATGCCGTGGCCCCA
CACTGCCAGTGGGTCTTTACTCCACACACCTCTCCCCCATGAATATTAGGCAACCCCATTTTCCCCATGA
CATTGTTGTAGTGTCCTCCCCTCAGGCTCTTGTTGCCTTGTGTACTGTGTACCCTTGTTTGGCATTTGCA
TGATGTACCAGTCATTAAACTGGTTGGCTGC
nt 1 - nt 731 of X15481.1
Notes:The probe used in this study by Mallo, 1997 [PMID:9142993] was described as "a 750-bp cDNA clone containing the entire coding sequence (Stoner & Gudas, 1989 [PMID:2538228] )". Although Mallo describes the sequence as being 750-bp, the entire sequence given by Stoner and Gudas (X115481) is 731-bp.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:(C57BL/6 x DBA/2)F1
Age:9.2 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Mallo, 1997 [PMID:9142993] . Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1997.8519] [ PMID:9142993] Mallo M 1997 Retinoic acid disturbs mouse middle ear development in a stage-dependent fashion. Dev Biol (184):175-86
 [ PMID:2538228] Stoner CM, Gudas LJ 1989 Mouse cellular retinoic acid binding protein: cloning, complementary DNA sequence, and messenger RNA expression during the retinoic acid-induced differentiation of F9 wild type and RA-3-10 mutant teratocarcinoma cells. Cancer Res (49):1497-504
Links:MGI:1340228 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI