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EMAGE:3347

Adrbk1 adrenergic receptor kinase, beta 1 ( MGI:87940)
TS20 (12.5 dpc)
in situ hybridisation

Data Images
EMAGE:3347 EMAGE:3347 EMAGE:3347
Fig 2G Sefton et al, 2000 [PMID:11044616] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00441-X] Mech Dev 98: 127-31, Sefton M; Blanco MJ; Penela P; Mayor F; Nieto MA, Expression of the G protein-coupled receptor kinase 2 during early mouse embryogenesis Copyright 2000 Fig 2F Sefton et al, 2000 [PMID:11044616] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00441-X] Mech Dev 98: 127-31, Sefton M; Blanco MJ; Penela P; Mayor F; Nieto MA, Expression of the G protein-coupled receptor kinase 2 during early mouse embryogenesis Copyright 2000 Fig 2H Sefton et al, 2000 [PMID:11044616] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00441-X] Mech Dev 98: 127-31, Sefton M; Blanco MJ; Penela P; Mayor F; Nieto MA, Expression of the G protein-coupled receptor kinase 2 during early mouse embryogenesis Copyright 2000

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Section G is taken from the wholemount sample in F. Image H is a high power view of G showing expression in the skin. Image annotation: id - interdigital region
Expression Pattern Description
Spatial Annotation:
EMAGE:3347EMAGE:3347Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3347_voxel_strong_3D_1.wlz
3347_voxel_moderate_3D_1.wlz
3347_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3347_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
interdigital region between forelimb digits 1 and 2
detected detected
regionalExpression is in undifferentitated cells
interdigital region between forelimb digits 2 and 3
detected detected
regionalExpression is in undifferentitated cells
interdigital region between forelimb digits 3 and 4
detected detected
regionalExpression is in undifferentitated cells
interdigital region between forelimb digits 4 and 5
detected detected
regionalExpression is in undifferentitated cells
hand rest of epithelium
detected detected
regionalExpression is in the skin
forelimb digit 1 epithelium
detected detected
regionalExpression is in the skin
forelimb digit 2 epithelium
detected detected
regionalExpression is in the skin
forelimb digit 3 epithelium
detected detected
regionalExpression is in the skin
forelimb digit 4 epithelium
detected detected
regionalExpression is in the skin
forelimb digit 5 epithelium
detected detected
regionalExpression is in the skin
interdigital region between forelimb digits 1 and 2 epithelium
detected detected
regionalExpression is in the skin
interdigital region between forelimb digits 2 and 3 epithelium
detected detected
regionalExpression is in the skin
interdigital region between forelimb digits 3 and 4 epithelium
detected detected
regionalExpression is in the skin
interdigital region between forelimb digits 4 and 5 epithelium
detected detected
regionalExpression is in the skin
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2386869
Entity Detected:Adrbk1, adrenergic receptor kinase, beta 1 ( MGI:87940)
Notes:Two Adrbk1 (GRK2) probes were used in this study by Sefton et al, 2000 [PMID:11044616] . They are described as "a NotI/SacI (540 bp) and a HindIII/SpHI (933bp) fragments subcloned from the complete mouse cDNA, which correspond to the amino and carboxy-terminal domains of the GRK2 protein, respectively". Editor's Note: it is unclear from the manuscript whether the two probes were mixed together to produce this result or if one or the other was used alone.
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:BALB/c
Age:12.5 dpc
Theiler Stage:TS20
Mutations:none (wild-type)
Preparation:sectioned wholemount
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
General Information
Authors:Sefton M, Blanco MJ, Penela P, Mayor F, Nieto MA (2000) [PMID:11044616] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00441-X] [ PMID:11044616] Sefton M, Blanco MJ, Penela P, Mayor F, Nieto MA 2000 Expression of the G protein-coupled receptor kinase 2 during early mouse embryogenesis. Mech Dev (98):127-31
Links:MGI:2386962 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI