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EMAGE:3349

Ambp alpha 1 microglobulin/bikunin ( MGI:88002)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:3349
Fig 1B, Sanchez et al 2002 [PMID:12204273] .Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(02)00202-2] Mech Dev 117: 293-8, Sanchez D; Martinez S; Lindqvist A; Akerstrom B; Falkenberg C, Expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in mouse embryogenesis. Copyright 2002

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotation: Arrow points to the liver primordium.
Expression Pattern Description
Spatial Annotation:
EMAGE:3349Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3349_wholemount_strong_3D_1.wlz
3349_wholemount_possible_3D_1.wlz
3349_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3349_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
biliary bud intrahepatic part
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:22400
Entity Detected:Ambp, alpha 1 microglobulin/bikunin ( MGI:88002)
Sequence:sense strand is shown

>MGI:22400
CCCACACTGAGCTGTGAGAGAGACATCAAAGACCAAAGACCACAGAGCCATGCAGGGTCTCAGGACCCTG
TTCCTGCTGCTGACTGCCTGCCTCGCTTCGAGGGCTGACCCTGCGTCAACACTGCCAGATATCCAGGTTC
AGGAGAACTTCAGTGAGTCCCGGATCTATGGAAAATGGTACAACCTGGCGGTGGGATCCACTTGCCCGTG
GCTGAGCCGCATTAAGGACAAGATGAGCGTGCAGACGCTGGTGCTGCAGGAGGGGGCGACAGAAACAGAG
ATCAGCATGACCAGTACTCGATGGCGGAGAGGTGTCTGTGAGGAGATCACTGGGGCGTACCAGAAGACGG
ACATCGATGGAAAGTTCCTCTACCACAAATCCAAATGGAACATAACCTTGGAATCCTATGTGGTCCACAC
CAACTATGACGAATATGCCATTTTCCTTACCAAGAAGTCCAGCCACCACCACGGGCTCACCATCACTGCC
AAGCTCTATGGTCGGGAGCCACAGCTGAGGGACAGCCTTCTGCAGGAGTTCAAGGATGTGGCCCTGAATG
TGGGCATCTCTGAGAACTCCATCATTTTTATGCCTGACAGAGGGGAATGTGTCCCTGGGGATCGGGAGGT
GGAGCCCACGTCAATTGCCAGAGCCCGGCGGGCAGTGCTGCCCCAAGAGAGTGAGGGGTCAGGGACTGAG
CCACTAATAACTGGGACCCTCAAGAAAGAAGACTCCTGCCAGCTCAATTACTCAGAAGGCCCCTGCCTAG
GGATGCAAGAGAGGTATTACTACAACGGCGCTTCCATGGCCTGCGAGACCTTTCAATATGGGGGTTGCCT
AGGCAACGGCAACAACTTCATCTCTGAGAAGGACTGTCTGCAGACATGTCGGACCATAGCGGCCTGCAAT
CTCCCCATAGTCCAAGGCCCCTGCCGAGCCTTCATAAAGCTCTGGGCATTTGATGCAGCACAAGGGAAGT
GCATCCAATTCCACTACGGGGGCTGCAAAGGCAACGGCAACAAATTCTACTCTGAGAAGGAATGCAAAGA
GTACTGTGGAGTCCCTGGTGATGGGTACGAGGAACTGATACGCAGTTGAAGGTGCCAGTCTGCAAGCCAG
AGGGTAGCCACTGTTTGTCACAGCGCAGTCCAGCTTAGATGATCTGGACCCAAATAAAAC
nt 1 - nt 1180 of X68680.1
Notes:The probe used in this study by Sanchez et al 2002 [PMID:12204273] is described as being "synthesized from the entire mouse AMBP cDNA clone", which was "kindly donated by Dr. J.-P. Salier (Chan and Salier, 1993 [PMID:7689339] )". Editor's note: Sequence X68680.1 was submitted directly to GenBank from J. Salier and refers to the clone in Chan and Salier, 1993.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Sanchez D; Martinez S; Lindqvist A; Akerstrom B; Falkenberg C, 2002 [PMID:12204273] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(02)00202-2] [ PMID:12204273] Sanchez D, Martinez S, Lindqvist A, Akerstrom B, Falkenberg C 2002 Expression of the AMBP gene transcript and its two protein products, alpha(1)-microglobulin and bikunin, in mouse embryogenesis. Mech Dev (117):293-8
 [ PMID:7689339] Chan P, Salier JP 1993 Mouse alpha-1-microglobulin/bikunin precursor: cDNA analysis, gene evolution and physical assignment of the gene next to the orosomucoid locus. Biochim Biophys Acta (1174):195-200
Links:MGI:3040518 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI