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EMAGE:3363

Tubb3 tubulin, beta 3 class III ( MGI:107813)
TS17 (10.5 dpc)
immunohistochemistry

Data Images
EMAGE:3363 EMAGE:3363
Figure 8A of Mastick et al., 1997 [PMID:9169845] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright. Figure 8B of Mastick et al., 1997 [PMID:9169845] Copyright: This image is from Development and is displayed with the permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: cv, cerebral vesicle; hs, hemispheric sulcus. The enlarged area depicted in B is shown boxed in A. Image annotations: cv - cerebral vesicle; hs - hemispheric sulcus; B - rectangle indicated in figure A shows region shown in greaater detail in figure B.
Expression Pattern Description
Spatial Annotation:
EMAGE:3363EMAGE:3363Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3363_voxel_strong_3D_1.wlz
3363_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3363_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
regionalExpression detected in the ventral prosencephalon, cerebral vesicle and dorsal mesencephalon, prosomeres 1 and 2.
telencephalon
detected detected
regionalExpression detected in the ventral prosencephalon, cerebral vesicle and dorsal mesencephalon, prosomeres 1 and 2.
midbrain
detected detected
regionalExpression detected in the ventral prosencephalon, cerebral vesicle and dorsal mesencephalon, prosomeres 1 and 2.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1860227
Entity Detected:Tubb3, tubulin, beta 3 class III ( MGI:107813)
Notes:The anti-class-III beta-tubulin antisera used in this study by Mastick et al, 1997 [PMID:9169845] is described as the "TuJ1 antibody", "as previously described (Easter et al., 1993 [PMID:8423474] ). Easter et al, in turn refer to Lee et al, 1990 [PMID:2257630] who describe TuJ1 as a "mouse monoclonal antibody" and state that "the TuJ1 secreting clone was isolated from hybrids produced by the fusion of SP2/0 myeloma cells with splenocytes of Balb/c mice immunized with taxol-assembled microtubules". They go on to say that using three independent methods, they have established that the antibody only binds to the tubulin beta-III C-terminus. Editor's Note: In another paper from 1993 by Lee et al [PMID:2402501] the authors specifically state that "TuJ1 reacts with an epitope located within the last 15 C-terminal residues (isotype-defining sequence)". The corresponding 15 amino acid epitope sequence is aa436 -aa 450 of NP_075768.1.
Antibody Type:monoclonal
Raised In:mouse
Catalogue Number:TuJ1
Specimen
Organism:mouse
Strain:FVB
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:cryosection
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Mastick GS, Davis NM, Andrew GL, Easter SS, 1997 [PMID:9169845] . Indexed by GXD, Spatially Mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9169845] Mastick GS, Davis NM, Andrew GL, Easter SS 1997 Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain. Development (124):1985-97
 [ PMID:8423474] Easter SS, Ross LS, Frankfurter A 1993 Initial tract formation in the mouse brain. J Neurosci (13):285-99
 [ doi:10.1002/cm.970170207] [ PMID:2257630] Lee MK, Tuttle JB, Rebhun LI, Cleveland DW, Frankfurter A 1990 The expression and posttranslational modification of a neuron-specific beta-tubulin isotype during chick embryogenesis. Cell Motil Cytoskeleton (17):118-32
 [ doi:10.1073/pnas.87.18.7195] [ PMID:2402501] Lee MK, Rebhun LI, Frankfurter A 1990 Posttranslational modification of class III beta-tubulin. Proc Natl Acad Sci U S A (87):7195-9
Links:MGI:1927527 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI