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EMAGE:3373

Pax6 paired box gene 6 ( MGI:97490)
TS15 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:3373 EMAGE:3373
Figure 1B of Mastick et al., 1997 [PMID:9169845] . Copyright: This image is from Mastick GS, Development 1997 May;124(10):1985-97, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright. Figure 1C of Mastick et al., 1997 [PMID:9169845] . Copyright: This image is from Mastick GS, Development 1997 May;124(10):1985-97, and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Note: p1,p2, prosomeres 1,2; cv, cerebral vesicle; m, mesencephalon.
Expression Pattern Description
Spatial Annotation:
EMAGE:3373EMAGE:3373Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3373_voxel_strong_3D_1.wlz
3373_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3373_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
Expression is detected in prosomeres 1,2 and 3 (3 is data not shown) and the cerebral vesicle.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1859757
Entity Detected:Pax6, paired box gene 6 ( MGI:97490)
Antigen:sense strand is shown

>MGI:1859757
QVPGSEPDMSQYWPRLQ
aa 420 - aa 436 of NP_038655.1
Notes:The anti-Pax6 antisera used in this study by Mastick et al., 1997 [PMID:9169845] is indicated as that originally described by Davis & Reed, 1996 [PMID:8756438] , which was raised against a synthetic peptide "corresponding to the 17 C-terminal residues of the mouse Pax-6 protein (H2N-QVPGSEPDMSQYWPRLQ-COOH)".
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Embedding:cryosection
Secondary Antibody:biotinylated anti-rabbit
Labelled with:avidin conjugated horse radish peroxidase
Visualisation method:purple VIP (Vector)
General Information
Authors:Mastick GS; Davis NM; Andrew GL; Easter SS Jr, 1997 [PMID:9169845] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9169845] Mastick GS, Davis NM, Andrew GL, Easter SS 1997 Pax-6 functions in boundary formation and axon guidance in the embryonic mouse forebrain. Development (124):1985-97
 [ PMID:8756438] Davis JA, Reed RR 1996 Role of Olf-1 and Pax-6 transcription factors in neurodevelopment. J Neurosci (16):5082-94
Links:MGI:1927486 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI