Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3441

Htr2b 5-hydroxytryptamine (serotonin) receptor 2B ( MGI:109323)
TS14 (9 dpc)
in situ hybridisation

Data Images
EMAGE:3441
Figure 2B of Choi et al., 1997 [PMID:9165122] . Copyright: This image is from Choi, et al., Development 124:1745-1755 (1997), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Scale bar, 100 um. b, cephalic neural plate region; h, heart region; s, somites; *, labelling of the caudal region including hindgut which was sometimes observed with sense probe and therefore may be non-specific.
Expression Pattern Description
Spatial Annotation:
EMAGE:3441Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3441_wholemount_strong_3D_1.wlz
3441_wholemount_moderate_3D_1.wlz
3441_wholemount_possible_3D_1.wlz
3441_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3441_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
neural tube
strong strong
heart
strong strong
embryo
strong strong
regionalExpression persisted in the cephalic region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1332611
Entity Detected:Htr2b, 5-hydroxytryptamine (serotonin) receptor 2B ( MGI:109323)
Notes:The Htr2b probe used in this study by Choi et al., 1997 [PMID:9165122] was a "whole 5-HT2B [Htr2b] cDNA antisense probe".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Choi DS; Ward SJ; Messaddeq N; Launay JM; Maroteaux L, 1997 [PMID:9165122] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:9165122] Choi DS, Ward SJ, Messaddeq N, Launay JM, Maroteaux L 1997 5-HT2B receptor-mediated serotonin morphogenetic functions in mouse cranial neural crest and myocardiac cells. Development (124):1745-55
Links:MGI:1332676 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI