EMAGE mouse gene expression spatial database

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EMAGE:3445

Cfc1 cripto, FRL-1, cryptic family 1 ( MGI:109448)
TS10 (LS Downs & Davies)
in situ hybridisation

Data Images


Figure 8A (right hand embryo) of Shen et al., 1997 [PMID:9053319] .Copyright: This image is from Shen et al., Development 124:429-442 (1997), and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

3445_wholemount_strong_3D_1.wlz

3445_wholemount_moderate_3D_1.wlz

3445_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:3445_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

primitive streak

detected

regional

Cryptic expression begins with localized staining at the anterior end of the primitive streak

embryo mesoderm

detected

spotted

Cryptic is expressed by scattered cells in the mesodermal wings emerging from the primitive streak

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:1334279
Entity Detected:	Cfc1, cripto, FRL-1, cryptic family 1 ( MGI:109448)
Sequence:	sense strand is shown >MGI:1334279 CAGGACTGTATAGGGTCAGCACTTCCAGCCTGGTGGTTCAGAGCTCCTGACCTGAGAGGGCTTCAACACC TGGACTCCAGGATCTTCCTTTAACCCTGCTGTCTCTGGTCCAGGCAGAGGGCAGAGACATCTTCATCTTG CAAGACTGTGCATCCTGTAACCTGCTATAGTGATTCCAAGACCTGGAGTAAAGGGTGCCTCCGGGGCTAG GATATTTGAGTTTCAACTTCTGTGGTCATCGATCCCCAAGCACAGATGAGAGCGAACTCACCAACCCAGG GTATCAGTTTGAAAATGCATCAAGCCAGGCCTCTGTTTTTGGTGACTGTCGCGCTGCAGCTCATCGGTCT GGGATACAGTTATCAGAGCGAAGGAGATGGTGCCAGAGAAGTCAGCAATATCCTCTCTCCAGTGATCCCC GGGACGACACTGGACAGAACTCTGAGTAATTCCAGCAGAAAGAATGACATTCCGGAGGGGGCGCGCCTAT GGGATTCCCTTCCTGACTCCAGCACTTTGGGAGAGAGTGCAGTCCCTGTATCCCGCTGTTGCCACAATGG CGGCACCTGTGTTCTGGGCAGTTTCTGCGTGTGCCCTGCCTATTTCACTGGTCGCTATTGCGAGCACGAC CAGAGGCGCAGAGACTGTGGTGCCCTAGGGCATGGAGCTTGGACCCTGCACAGCTGCCGCCTATGCAGGT GCATCTTCTCAGCCCTGTACTGCCTCCCACACCAAACGTTCAGCCACTGTGACCTGAAAAGCTTCCTTTC TTCAGGCGCCAGAGGATCAAGAGAATGCAGCATCCCAAGCCTCCTCCTGCTGGTGCTCTGCCTCCTCCTG CAGGGTGTGGCTGGTAAGGGCTGAGGCTCCTAAGTGCGATGATAGACTCTCCTCCTGAGCTGTCACCCTT GATTACACCACAGTGTGCCAGCAAGAAAGCTGGGTGGTGGGCATCTGACTTGGTGTTGTGTCCTGTAAAT AACAGATTCACTGGAATATGCTGGATTCTCATGCTGTACAATAAAGAGGCTTAATGGTG
	nt 1 - nt 1039 of U57720.1
Notes:	The Cfc1 (cryptic) probe used is this study by Shen et al, 1997 [PMID:9053319] is described as the "entire cDNA". The cryptic cDNA sequence U57720.1 includes both UTRs and ORF sequence and was a direct submission to GenBank from Michael Shen.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	Swiss Webster
Age:	LS Downs & Davies
Theiler Stage:	TS10
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Staining procedure:

alkaline phosphatase + NBT/BCIP

General Information

Authors:	Shen MM; Wang H; Leder P, 1997 [PMID:9053319] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ PMID:9053319] Shen MM, Wang H, Leder P 1997 A differential display strategy identifies Cryptic, a novel EGF-related gene expressed in the axial and lateral mesoderm during mouse gastrulation. Development (124):429-42
Links:	MGI:1334280 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source