EMAGE mouse gene expression spatial database

EMAGE:3457

Csk c-src tyrosine kinase ( MGI:88537)
TS18 (10.5 dpc)
in situ hybridisation

Data Images


Fig 1K.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.04.009] Gene Expr Patterns 6: 94-101, Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Copyright 2005

Expression pattern clarity:
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Notes:

Image annotations: Arrows point to segmented expression in dorsal root ganglia, arrowhead indicates expression in the trigeminal ganglion.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

branchial arch

detected

trigeminal v ganglion

detected

dorsal root ganglion

detected

limb

detected

regional

Expression is in the distal limb buds.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3617170
Entity Detected:	Csk, c-src tyrosine kinase ( MGI:88537)
Sequence:	sense strand is shown >MGI:3617170 CCTGGCCATCCGGTACAGAATGTATTGCCAAGTACAACTTCCATGGCACTGCTGAGCAAGACCTTCCCTT CTGCAAAGGAGATGTGCTCACCATCGTGGCTGTCACCAAGGACCCCAACTGGTACAAAGCCAAAAACAAA GTGGGCCGTGAGGGCATCATCCCAGCCAACTATGTCCAGAAGCGTGAGGGTGTGAAGGCAGGCACCAAAC TCAGCCTTATGCCCTGGTTCCACGGCAAGATCACACGGGAGCAGGCCGAGCGGCTTCTTTACCCACCAGA GACAGGCCTGTTCCTCGTGCGGGAAAGCACCAACTACCCTGGAGACTACACACTGTGTGTGAGCTGTGAG GGCAAGGTGGAGCACTACCGCATCATGTATCATGCGAGCAAGCTGAGCATTGATGAGGAGGTGTACTTTG AGAACCTCATGCAGCTGGTGGAGCACTACACCACAGATGCCGATGGACTCTGCACTCGCCTCATCAAACC AAAGGTCATGGAGGGCACCGTGGCGGCCCAGGATGAGTTCTACCGCAGTGGCTGGGCACTGAACATGAAG GAACTGAAGCTGCTACAGACAATAGGGAAGGGGGAGTTTGGAGATGTGATGCTGGGG
	nt 268 - nt 884 of NM_007783.2
Notes:	The Csk probe used in this study by Machka et al, 2005 [PMID:15979417] is described as being "isolated and amplified from E10.5 embryonic total RNA by reverse transcription-PCR and cloned using the TopoTA Cloning Kit (Invitrogen). Primers were: Csk, 617 bp: 5'CCTGGCCATCCGGTACAGAA 3' and 5'CCCCAGCATCACATCTCCAA 3'."
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + BMpurple

General Information

Authors:	Machka C; Kersten M; Zobawa M; Harder A; Horsch M; Halder T; Lottspeich F; Hrabe de Angelis M; Beckers J, 2005 [PMID:15979417] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2005.04.009] [ PMID:15979417] Machka C, Kersten M, Zobawa M, Harder A, Horsch M, Halder T, Lottspeich F, Hrabe de Angelis M, Beckers J 2005 Identification of Dll1 (Delta1) target genes during mouse embryogenesis using differential expression profiling. Gene Expr Patterns (6):94-101
Links:	MGI:3617194 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source