EMAGE mouse gene expression spatial database

EMAGE:3609

Tle4 transducin-like enhancer of split 4, homolog of Drosophila E(spl) ( MGI:104633)
TS11 (EHF Downs & Davies)
in situ hybridisation

Data Images


Fig2A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/0925-4773(96)00582-5] Mech Dev 59: 73-87, Koop KE; MacDonald LM; Lobe CG, Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development. Copyright 1996

Expression pattern clarity:
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Notes:

Image annotations: arrow points to Grg4 (Tle4) transcripts in the lateral mesoderm; arrowhead points to position of the node.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo mesoderm

detected

regional

Expression is in lateral mesoderm

amnion

detected

extraembryonic component

detected

regional

Authors state expression is in the migrating extraembryonic mesoderm.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3044199
Entity Detected:	Tle4, transducin-like enhancer of split 4, homolog of Drosophila E(spl) ( MGI:104633)
Sequence:	sense strand is shown >MGI:3044199 AGAGCTGACATCCTCAGCCCCTGCCTGCTATGCTCTGGCCATCAGCCCCGACTCCAAGGTCTGCTTCTCA TGCTGCAGCGACGGTAACATCGCAGTGTGGGATCTGCACAACCAGACTCTGGTGAGGCAATTCCAGGGGC ACACAGATGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACAGGTGGTTTGGACAA CACTGTGAGGTCCTGGGACCTGCGTGAAGGGCGGCAGCTGCAGCAACATGACTTCACCTCTCAGATCTTT TCATTGGGCTATTGCCCAACTGGAGAGTGGCTTGCAGTGGGGATGGAGAATAGCAATGTGGAAGTATTGC ATGTCACCAAACCAGACAAATACCAGTTGCATCTTCATGAGAGCTGTGTGCTGTCACTCAAGTTTGCCCA CTGTGGCAAATGGTTTGTAAGCACTGGAAAGGACAACCTTCTGAATGC
	nt 2284 - nt 2751 of NM_011600.2
Notes:	The DNA template used for generation of the Tle4 (Grg4) probe used in this study by Koop et al, 1996 [PMID:8892234] "was the 459 bp sequence from the WD40 domain obtained by PCR". This was amplified using the following primers: 5'-TCCTCGGCTCCAGCCTGTTA-3' and 5'-GGCATTGAGAAGGTTGTCTT-3', to produce the 459 bp fragment. Editor's Note: the are a considerable number of base-pair mis-matches between these primer sequences and the Tle4 cDNA RefSeq NM_011600.2. Manual alignment was used to find the start and end co-ordinates within NM_011600.2.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	4% paraformaldehyde
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Koop KE; MacDonald LM; Lobe CG (1996) [PMID:8892234] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/0925-4773(96)00582-5] [ PMID:8892234] Koop KE, MacDonald LM, Lobe CG 1996 Transcripts of Grg4, a murine groucho-related gene, are detected in adjacent tissues to other murine neurogenic gene homologues during embryonic development. Mech Dev (59):73-87
Links:	MGI:3044222 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source