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EMAGE:3627

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS14 (9.5 dpc)
immunohistochemistry

Data Images
EMAGE:3627
Fig 2DCopyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1995.1099] Dev Biol 168: 514-28, Leonard L; Horton C; Maden M; Pizzey JA, Anteriorization of CRABP-I expression by retinoic acid in the developing mouse central nervous system and its relationship to teratogenesis. Copyright 1995

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: arrowheads indicate streams of neural crest.
Expression Pattern Description
Spatial Annotation:
EMAGE:3627Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3627_wholemount_strong_3D_1.wlz
3627_wholemount_moderate_3D_1.wlz
3627_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3627_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme derived from neural crest
detected detected
regionalExpression is in streams of neural crest that is migrating from rhombomeres 4 and 6, populating the branchial arches and heart.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:antibody
Identifier:MGI:1340376
Entity Detected:Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Notes:The anti-CRABP1 antibody used in this study by Leonard et al., 1995 [PMID:7729586] is described as "an affinity-purified rabbit polyclonal antibody as previously described (Maden et al., 1992 [PMID:1351399] )". Maden et al 1992, state that the antibody was "raised against a synthetic peptide corresponding to bovine CRABP1", that the characteristics of this antibody were previously described by Eriksson et al, 1987 [PMID:2826496] and that it was also used previously as an immunocytochemical reagent in mouse embryos (Denckner et al, 1990 [PMID:1966832] ). Eriksson et al, 1987 generated antisera against three peptides: residues 68-81 (GEGFEEETVDGRKC); 81-94 (CRSLPTWENENKIH) and 95-103 (CTQTLLEGDGPKT) of bovine CRABP1. Denckner et al, 1990 used two of these in their studies (the two raised against residues "69-84 and 95-107 of bovine CRABP1" and state that they gave identical results. Editors Note: It is not clear which of these antisera have been used in the study by Leonard et al., 1995 [PMID:7729586] .
Antibody Type:polyclonal
Raised In:rabbit
Specimen
Organism:mouse
Strain:involves: Swiss * TO
Age:9.5 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:methanol
Secondary Antibody:anti rabbit-biotinylated
Tertiary Antibody:streptavidin-HRP
Labelled with:horse radish peroxidase
Visualisation method:DAB
General Information
Authors:Leonard L; Horton C; Maden M; Pizzey JA, 1995 [PMID:7729586] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1995.1099] [ PMID:7729586] Leonard L, Horton C, Maden M, Pizzey JA 1995 Anteriorization of CRABP-I expression by retinoic acid in the developing mouse central nervous system and its relationship to teratogenesis. Dev Biol (168):514-28
 [ doi:10.1016/0925-4773(92)90011-8] [ PMID:1351399] Maden M, Horton C, Graham A, Leonard L, Pizzey J, Siegenthaler G, Lumsden A, Eriksson U 1992 Domains of cellular retinoic acid-binding protein I (CRABP I) expression in the hindbrain and neural crest of the mouse embryo. Mech Dev (37):13-23
 [ doi:10.1002/jcp.1041330308] [ PMID:2826496] Eriksson U, Hansson E, Nordlinder H, Busch C, Sundelin J, Peterson PA 1987 Quantitation and tissue localization of the cellular retinoic acid-binding protein. J Cell Physiol (133):482-90
 [ PMID:1966832] Dencker L, Annerwall E, Busch C, Eriksson U 1990 Localization of specific retinoid-binding sites and expression of cellular retinoic-acid-binding protein (CRABP) in the early mouse embryo. Development (110):343-52
Links:MGI:1340408 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI