EMAGE mouse gene expression spatial database

EMAGE:3628

Crabp1 cellular retinoic acid binding protein I ( MGI:88490)
TS12 (8.5 dpc)
immunohistochemistry

Data Images


Fig 2ECopyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1995.1099] Dev Biol 168: 514-28, Leonard L; Horton C; Maden M; Pizzey JA, Anteriorization of CRABP-I expression by retinoic acid in the developing mouse central nervous system and its relationship to teratogenesis. Copyright 1995	Fig 2FCopyright: Reprinted with permission from Elsevier from [ [doi:10.1006/dbio.1995.1099] Dev Biol 168: 514-28, Leonard L; Horton C; Maden M; Pizzey JA, Anteriorization of CRABP-I expression by retinoic acid in the developing mouse central nervous system and its relationship to teratogenesis. Copyright 1995

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Image annotation: POS - preotic sulcus; arrowheads indicate extent of Crabp1 expression.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

Download individual expression domains:

Download all expression domains:

Find spatially similar wholemount expression patterns:

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

future hindbrain

detected

regional

Expression is in a stripe in the future rhombencephalon when the neural folds are still open.

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	antibody
Identifier:	MGI:1340376
Entity Detected:	Crabp1, cellular retinoic acid binding protein I ( MGI:88490)
Notes:	The anti-CRABP1 antibody used in this study by Leonard et al., 1995 [PMID:7729586] is described as "an affinity-purified rabbit polyclonal antibody as previously described (Maden et al., 1992 [PMID:1351399] )". Maden et al 1992, state that the antibody was "raised against a synthetic peptide corresponding to bovine CRABP1", that the characteristics of this antibody were previously described by Eriksson et al, 1987 [PMID:2826496] and that it was also used previously as an immunocytochemical reagent in mouse embryos (Denckner et al, 1990 [PMID:1966832] ). Eriksson et al, 1987 generated antisera against three peptides: residues 68-81 (GEGFEEETVDGRKC); 81-94 (CRSLPTWENENKIH) and 95-103 (CTQTLLEGDGPKT) of bovine CRABP1. Denckner et al, 1990 used two of these in their studies (the two raised against residues "69-84 and 95-107 of bovine CRABP1" and state that they gave identical results. Editors Note: It is not clear which of these antisera have been used in the study by Leonard et al., 1995 [PMID:7729586] .
Antibody Type:	polyclonal
Raised In:	rabbit

Specimen

Procedures

General Information

Authors:	Leonard L; Horton C; Maden M; Pizzey JA, 1995 [PMID:7729586] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1006/dbio.1995.1099] [ PMID:7729586] Leonard L, Horton C, Maden M, Pizzey JA 1995 Anteriorization of CRABP-I expression by retinoic acid in the developing mouse central nervous system and its relationship to teratogenesis. Dev Biol (168):514-28
	[ doi:10.1016/0925-4773(92)90011-8] [ PMID:1351399] Maden M, Horton C, Graham A, Leonard L, Pizzey J, Siegenthaler G, Lumsden A, Eriksson U 1992 Domains of cellular retinoic acid-binding protein I (CRABP I) expression in the hindbrain and neural crest of the mouse embryo. Mech Dev (37):13-23
Links:	MGI:1340408 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source