| Type: | in situ hybridisation probe |
| Identifier: | MGI:1277367 |
| Entity Detected: | Alx3, aristaless-like homeobox 3 ( MGI:1277097) |
| Sequence: | sense strand is shown
>MGI:1277367
CCATGGAAATGTGGCTGGCTTCATGGGAGTGCCAGCCTCCCCTGCAGCCCACCCTGGCATCTATTCCATC
CATGGCTTCCCTCCTGCCCTGGGAGGGCACAGCTTTGAGCCTTCTCCGGATGGTGACTACAAGTCCCCAA
GCCTCGTTTCACTCAGGATGAAGCCCAAAGAGCCCCCTGGCCTGCTGAACTGGACCACGTGATGGGTTAC
ATGGGCACAGACTGAGCTGTCCACTTCTCTTCCTGCTCTGACCTGCTCCTAGCCCATCTCTACCTGGACA
CCAAACAGCAAACCCTTTTACCTCCATAACCTGGGTGGTTCTGGAAGCTTGGAAGAGGAGCTGGGACCCT
CAGCCTCCTGCAGGGAACAGGCTACAGGGTCTTATTTCTTAAAATAGGTGGGGCTGCCTAGGAGACAGGG
ACTTCAAAGCAGGTAGGACTCCTCCTTATAAAATGGCTCCCCTCATTTAAATTGGGCTGGAGAAAAGGTG
ACAGATGCCCCCTGTTTTCCAAAGACTGTTTCTAAGTCCAGGGAGCCCAGGAAGATGGGAGGAGGGTGCA
GACTAAACAGGGAGGCGCTCTCTGTGGTTCTTAGGGCTGGCCTCACTTTTGCCTTCCTACCACCAGGGGG
CGGTACGCTCAGCTCCTGGGACTGACAAGATCCCAGACGGTGAAGGTCAGCTCAATAATAGGTGGGGGGG
TCTCCTCTGTTCCAAGCATCCCTCACTTCTGCCCTGTGCTAGTCTACAGAGATACTATAAAGTCATTTTC
TCCAAGTAGGGAGAAGAAATTCCACCAAACATTAAATATTTGGATTTGCTTTCATGGGATCAGGGATATG
CCATGAAAACCATATTGGACATCGATCAAAATAAAAAAAAAATTTTTTTTCC
|
| | nt 979 - nt 1870 of U96109.1 |
| Notes: | The Alx3 probe used in this study by Beverdam and Meijlink (2001) [PMID:11520673] is indicated as that used by ten Berge et al (1998) [PMID:9676189] i.e. "A plasmid consisting of a 1.856-kb Alx3 cDNA cloned in pEXLox (Novagen) was cut with NcoI and transcribed with Sp6 polymerase. This results in an antisense RNA complementary to the 3'-untranslated region of the mRNA and the part encoding the C-terminal 66 amino acids."
Editors note: Alx3 sequence U96109.1 was submitted to GenBank by ten Berge. Restriction cutting of U96109.1 with NcoI identifies 3 sites. A cut at the most 3' site (position 1048) would yield a fragment containing the 3'UTR and the most C-terminal 44 amino acids, whereas a cut at the second most 3' site (position 979) would yield a fragment containing the 3'UTR and the most C-terminal 66 amino acids as ten Berge states in their description. As such the sequence from 979 - the 3' end of the clone has been included here as the probe sequence. |
| Chemistry: | RNA |
| Strand: | antisense |
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