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EMAGE:3873

Dkk1 dickkopf homolog 1 (Xenopus laevis) ( MGI:1329040)
TS15 (10-10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3873
Fig8A.Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00138-0] Mech Dev 87: 45-56, Monaghan AP; Kioschis P; Wu W; Zuniga A; Bock D; Poustka A; Delius H; Niehrs C, Dickkopf genes are co-ordinately expressed in mesodermal lineages. Copyright 1999

Expression pattern clarity: three stars
Find spatially similar expression patterns: Find spatially similar patterns
Notes:
Image annotations: rp - Rathke's pouch, b - branchical arch, arrowheads - anterior and posterior limits of expression domain.
Expression Pattern Description
Spatial Annotation:
EMAGE:3873EMAGE:3873Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
3D mappingspatial mapping

View mapped 3D expression image EMAGE genex expression entry
Download individual expression domains:
3873_voxel_strong_3D_1.wlz
3873_voxel_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3873_all_domains.zip
Find spatially similar expression patterns: EMAGE spatially similar patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
diencephalon
detected detected
regionalExpression is present in a small region of the ventral diencephalon.
telencephalon
detected detected
regionalExpression is present in a small region.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1329444
Entity Detected:Dkk1, dickkopf homolog 1 (Xenopus laevis) ( MGI:1329040)
Sequence:sense strand is shown

>MGI:1329444
GACCCACGCGTCCGTGCCTGTTTGCGTCCTTCGGAGATGATGGTTGTGTGTGCACCGGCAGCTGTCCGGT
TCTTGGCCGTGTTTACAATGATGGCTCTCTGCAGCCTCCCTCTGCTAGGAGCCAGTGCCACCTTGAACTC
AGTTCTCATCAATTCCAACGCGATCAAGAACCTGCCCCCACCGCTGGGTGGTGCTGGGGGGCAGCCGGGC
TCTGCTGTCAGTGTGGCGCCGGGAGTTCTCTATGAGGGCGGGAACAAGTACCAGACTCTTGACAACTACC
AGCCCTACCCTTGCGCTGAAGATGAGGAGTGCGGCTCTGACGAGTACTGCTCCAGCCCCAGCCGCGGGGC
AGCCGGCGTCGGAGGTGTACAGATCTGTCTGGCTTGCCGAAAGCGCAGGAAGCGCTGCATGACGCACGCT
ATGTGCTGCCCCGGGAACTACTGCAAAAATGGAATATGCATGCCCTCTGACCACAGCCATTTTCCTCGAG
GGGAAATTGAGGAAAGCATCATTGAAAACCTTGGTAATGACCACAACGCCGCCGCGGGGGATGGATATCC
CAGAAGAACCACACTGACTTCAAAAATATATCACACCAAAGGACAAGAAGGCTCCGTCTGCCTCCGATCA
TCAGACTGTGCCGCAGGGCTGTGTTGTGCAAGACACTTCTGGTCCAAGATCTGTAAACCTGTCCTTAAAG
AAGGTCAGGTGTGCACCAAGCACAAACGGAAAGGCTCCCACGGGCTGGAGATATTCCAGCGCTGTTACTG
CGGGGAAGGCCTGGCTTGCAGGATACAGAAAGATCACCATCAAGCCAGCAATTCTTCTAGGCTCCACACC
TGCCAGAGACACTAAACCGACAGTCTAAATATGATGGACTCTTTTTATCTAATATATGCTACGAAAATCC
TTTATGATTTGTCAGCTCAATCCCAAGGATGTAGGAATCTTCAGTGTGTAATTAAGCATTCCGACAATAC
TTTCCAAAAGCTCTGGAGTGTAAGGACTTTGTTTCTTGATGGAACTCCCCTGTGATTGCAGTAAATTACT
GTGTTGTAAATCCTCAGTGTGGCACTTACCTGTAAATGCAGCAAAACTTTTAATTATTTTTCTAGAGGTG
TGGTACATTGCCTTGTTTCTCTTGCATGTAAATTTTTTTTGTACACGGTTGATTGTCTTGACTCATAAAT
ATTCTATATTGGAGTAGAAAAAAAAAAAAAAAAAAA
nt 1 - nt 1226 of AF030433.1
Notes:The Dkk1 probe used in this study by Monaghan et al, 1999 [PMID:10495270] is described as being transcribed from "full length dkk-1 (Glinka et al., 1998 [PMID:9450748] )". Glinka et al generated an in situ hybridisation probe from a clone that was isolated from a mouse embryonic day 9 cDNA library by the RZPD (clone MPMGc559P2481Q3). AF030433.1 is the accompanying GenBank entry for the insert sequence of this clone.
Chemistry:RNA
Strand:antisense
Label:S35
Specimen
Organism:mouse
Strain:NMRI
Age:10-10.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:4% paraformaldehyde
Embedding:paraffin
Staining procedure:autoradiography
General Information
Authors:Monaghan AP; Kioschis P; Wu W; Zuniga A; Bock D; Poustka A; Delius H; Niehrs C, 1999. [PMID:10495270] . Indexed by GXD, Spatially mapped by EMAGE
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00138-0] [ PMID:10495270] Monaghan AP, Kioschis P, Wu W, Zuniga A, Bock D, Poustka A, Delius H, Niehrs C 1999 Dickkopf genes are co-ordinately expressed in mesodermal lineages. Mech Dev (87):45-56
 [ doi:10.1038/34848] [ PMID:9450748] Glinka A, Wu W, Delius H, Monaghan AP, Blumenstock C, Niehrs C 1998 Dickkopf-1 is a member of a new family of secreted proteins and functions in head induction. Nature (391):357-62
Links:MGI:3603181 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI