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EMAGE:3969

Otx1 orthodenticle homolog 1 (Drosophila) ( MGI:97450)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3969
Figure 3L of Raft et al., 2004 [PMID:15084464] . Copyright: This image is from Raft S, Development 2004;131():1801-1812 and is displayed with the permission of The Company of Biologists Limited who owns the Copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:3969Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3969_wholemount_strong_3D_1.wlz
3969_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3969_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
endolymphatic appendage epithelium
detected detected
rest of otocyst epithelium
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3040958
Entity Detected:Otx1, orthodenticle homolog 1 (Drosophila) ( MGI:97450)
Notes:The Otx1 probe used in this study by Raft et al., 2004 [PMID:15084464] was a "PstI/SacI cDNA fragment". Editor's note: there are two possible PstI/SacI fragments that can be generated from the Otx1 cDNA RefSeq NM_011023.3: nt 636(SacI)-1026(PstI) or 1026(PstI)-1719(SacI). Unable to determine which was used as the probe.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 129/Sv * C57BL/6J * FVB * SJL
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Raft S; Nowotschin S; Liao J; Morrow BE, 2004 [PMID:15084464] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1242/dev.01067] [ PMID:15084464] Raft S, Nowotschin S, Liao J, Morrow BE 2004 Suppression of neural fate and control of inner ear morphogenesis by Tbx1. Development (131):1801-12
Links:MGI:3044712 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI