Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:3970

Pax3 paired box gene 3 ( MGI:97487)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:3970
Figure 5E of Wilson-Rawls et al., 1999 [PMID:10556048] . Copyright: This image is from Wilson-Rawls et al. Development 126:5217-5229 (1999), and is displayed with the permission of The Company of Biologist Limited who owns the Copyright.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Expression of Pax3 in dorsomedial and dorsolateral lips of dermomyotome are denoted by white and red arrowheads, respectively. White arrow marks Pax3-positive cells in limb muscle primordia.
Expression Pattern Description
Spatial Annotation:
EMAGE:3970Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
3970_wholemount_strong_3D_1.wlz
3970_wholemount_moderate_3D_1.wlz
3970_wholemount_possible_3D_1.wlz
3970_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:3970_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
regionalAs somites mature, Pax3 expression becomes more restricted to the dermomyotome, with highest levels in the dorsomedial and dorsolateral lips.
neural tube
detected detected
forelimb bud
detected detected
regionalPax3 transcripts were present in the proximal region of the forelimb buds (in muscle primordia).
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:19544
Entity Detected:Pax3, paired box gene 3 ( MGI:97487)
Notes:The Pax3 probe used in this study by Wilson-Rawls et al., 1999 [PMID:10556048] was originally described in Goulding et al., 1993 [PMID:8406486] . Editor's note: A number of Pax3 fragments which could have been used as template to generate the probe used in this assay are described in Goulding et al. Which one was used in this assay is unknown.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:10% Formalin
General Information
Authors:Wilson-Rawls J; Hurt CR; Parsons SM; Rawls A, 1999 [PMID:10556048] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10556048] Wilson-Rawls J, Hurt CR, Parsons SM, Rawls A 1999 Differential regulation of epaxial and hypaxial muscle development by paraxis. Development (126):5217-29
 [ doi:10.1006/geno.1993.1332] [ PMID:8406486] Goulding M, Sterrer S, Fleming J, Balling R, Nadeau J, Moore KJ, Brown SD, Steel KP, Gruss P 1993 Analysis of the Pax-3 gene in the mouse mutant splotch. Genomics (17):355-63
Links:MGI:1930196 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI