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EMAGE:4009

Mest mesoderm specific transcript ( MGI:96968)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4009 EMAGE:4009
Fig4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0012-1606(03)00116-7] Dev Biol 258: 91-104, Buttitta L; Tanaka TS; Chen AE; Ko MS; Fan CM, Microarray analysis of somitogenesis reveals novel targets of different WNT signaling pathways in the somitic mesoderm. Copyright 2003 Fig4B. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0012-1606(03)00116-7] Dev Biol 258: 91-104, Buttitta L; Tanaka TS; Chen AE; Ko MS; Fan CM, Microarray analysis of somitogenesis reveals novel targets of different WNT signaling pathways in the somitic mesoderm. Copyright 2003

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: psm - presomitic mesoderm; som - somites; dm - dermomyotome; scl - sclerotome; D-V axis - dorsoventral axis. Fig4B shows transverse (top) and horizontal (bottom) sections through the tail of embryos similar to that shown in Fig4A.
Expression Pattern Description
Spatial Annotation:
EMAGE:4009Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4009_wholemount_strong_3D_1.wlz
4009_wholemount_moderate_3D_1.wlz
4009_wholemount_weak_3D_1.wlz
4009_wholemount_possible_3D_1.wlz
4009_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4009_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
detected detected
Authors report broad expression in somites.
trunk mesenchyme
detected detected
regionalExpression in lateral plate mesoderm.
tail unsegmented mesenchyme
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3521677
Entity Detected:Mest, mesoderm specific transcript ( MGI:96968)
Notes:The Mest probe used in this study by Buttitta et al., 2003 [PMID:12781685] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:CD-1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Buttitta L; Tanaka TS; Chen AE; Ko MS; Fan CM, 2003 [PMID:12781685] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0012-1606(03)00116-7] [ PMID:12781685] Buttitta L, Tanaka TS, Chen AE, Ko MS, Fan CM 2003 Microarray analysis of somitogenesis reveals novel targets of different WNT signaling pathways in the somitic mesoderm. Dev Biol (258):91-104
Links:MGI:3521717 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI