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EMAGE:4023

Nppa natriuretic peptide type A ( MGI:97367)
TS16 (9.5-10.0 dpc)
in situ hybridisation

Data Images
EMAGE:4023 EMAGE:4023
Fig1H. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9753] Dev Biol 223: 266-78, Christoffels VM; Habets PE; Franco D; Campione M; de Jong F; Lamers WH; Bao ZZ; Palmer S; Biben C; Harvey RP; Moorman AF, Chamber formation and morphogenesis in the developing mammalian heart [published erratum appears in Dev Biol 2000 Sep 1;225(1):266] Copyright 2000 Fig1G. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.9753] Dev Biol 223: 266-78, Christoffels VM; Habets PE; Franco D; Campione M; de Jong F; Lamers WH; Bao ZZ; Palmer S; Biben C; Harvey RP; Moorman AF, Chamber formation and morphogenesis in the developing mammalian heart [published erratum appears in Dev Biol 2000 Sep 1;225(1):266] Copyright 2000

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: AVC= atrioventricular canal, IFT= inflow tract, OFT= outflow tract, IC= inner curvature, vIFT= ventral side of IFT, LV= left ventricle, RV= right ventricle, LA= left atrium, RA= right atrium. Figure 1H is a lateral view, Figure 1G is a frontal view.
Expression Pattern Description
Spatial Annotation:
EMAGE:4023Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4023_wholemount_strong_3D_1.wlz
4023_wholemount_moderate_3D_1.wlz
4023_wholemount_weak_3D_1.wlz
4023_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4023_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
heart atrium
detected detected
regionalExpression is restricted to the outer curvature of the heart tube.
primitive ventricle
detected detected
regionalExpression is restricted to the outer curvature of the heart tube.
atrioventricular canal
not detected not detected
homogeneous
outflow tract
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1927599
Entity Detected:Nppa, natriuretic peptide type A ( MGI:97367)
Notes:The Nppa (ANF) probe used in this study by Christoffels et al., 2000 [PMID:10882515] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:FVB
Age:9.5-10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + BMpurple
General Information
Authors:Christoffels VM; Habets PE; Franco D; Campione M; de Jong F; Lamers WH; Bao ZZ; Palmer S; Biben C; Harvey RP; Moorman AF, 2000 [PMID:10882515] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.9753] [ PMID:10882515] Christoffels VM, Habets PE, Franco D, Campione M, de Jong F, Lamers WH, Bao ZZ, Palmer S, Biben C, Harvey RP, Moorman AF 2000 Chamber formation and morphogenesis in the developing mammalian heart. Dev Biol (223):266-78
Links:MGI:1929929 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI