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EMAGE:4075

Tbx6 T-box 6 ( MGI:102539)
TS19 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:4075
Fig2L. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1996.0326] Dev Biol 180: 534-42, Chapman DL; Agulnik I; Hancock S; Silver LM; Papaioannou VE, Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Copyright 1996

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4075Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4075_wholemount_strong_3D_1.wlz
4075_wholemount_moderate_3D_1.wlz
4075_wholemount_possible_3D_1.wlz
4075_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4075_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
tail
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:33996
Entity Detected:Tbx6, T-box 6 ( MGI:102539)
Notes:The Tbx6 probe used in this study by Chapman et al., 1996 [PMID:8954725] is described as the linearised plasmid w32. w32 is described as containing approximately 1150 bp of the Tbx6 cDNA, including part of the T-box region, inserted at the EcoRI site of pBluescript II KS(-). The probe was hydrolyzed to an average size of 0.5 kb for whole-mount in situ analysis.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:Swiss Webster
Age:11.5 dpc
Theiler Stage:TS19
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Chapman DL; Agulnik I; Hancock S; Silver LM; Papaioannou VE, 1996 [PMID:8954725] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1996.0326] [ PMID:8954725] Chapman DL, Agulnik I, Hancock S, Silver LM, Papaioannou VE 1996 Tbx6, a mouse T-Box gene implicated in paraxial mesoderm formation at gastrulation. Dev Biol (180):534-42
Links:MGI:1340265 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI