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EMAGE:4078

Tbx5 T-box 5 ( MGI:102541)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4078
Fig2H. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.1999.9298] Dev Biol 211: 100-8, Bruneau BG; Logan M; Davis N; Levi T; Tabin CJ; Seidman JG; Seidman CE, Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome. Copyright 1999

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: fl = forelimb bud, r = retina, a = atrium, lv = left ventricle.
Expression Pattern Description
Spatial Annotation:
EMAGE:4078Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4078_wholemount_strong_3D_1.wlz
4078_wholemount_moderate_3D_1.wlz
4078_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4078_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
forelimb bud
detected detected
eye
detected detected
primitive ventricle
detected detected
regionalExpression is restricted to the left side of the ventricles.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1340309
Entity Detected:Tbx5, T-box 5 ( MGI:102541)
Notes:The Tbx5 probe used in this study by Bruneau et al., 1999 [PMID:10373308] is of undefined sequence.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:(Swiss Webster x C57BL/6)F1
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase
General Information
Authors:Bruneau BG; Logan M; Davis N; Levi T; Tabin CJ; Seidman JG; Seidman CE, 1999 [PMID:10373308] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.1999.9298] [ PMID:10373308] Bruneau BG, Logan M, Davis N, Levi T, Tabin CJ, Seidman JG, Seidman CE 1999 Chamber-specific cardiac expression of Tbx5 and heart defects in Holt-Oram syndrome. Dev Biol (211):100-8
Links:MGI:1340489 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI