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EMAGE:4083

Nog noggin ( MGI:104327)
TS15 (9.5 dpc)
in situ reporter

Data Images
EMAGE:4083
Fig1B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0479] Dev Biol 240: 457-73, Stottmann RW; Anderson RM; Klingensmith J, The BMP antagonists Chordin and Noggin have essential but redundant roles in mouse mandibular outgrowth. Copyright 2001

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: BA1 and white arrow indicates first Branchial Arch; Arrowheads indicate expression in the dorsal Neural Tube.
Expression Pattern Description
Spatial Annotation:
EMAGE:4083Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4083_wholemount_strong_3D_1.wlz
4083_wholemount_moderate_3D_1.wlz
4083_wholemount_weak_3D_1.wlz
4083_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4083_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch mandibular component mesenchyme
detected detected
single cellExpression is detected in a punctate pattern in the initial swelling (mesenchyme) of the 1st Branchial Arch suggesting migrating neural crest cells.
1st branchial arch mandibular component ectoderm
detected detected
regionalExpression detected in the dorsal neural tube in a Rhombomere specific pattern and the pharyngeal/caudal mandibular ectoderm.
neural tube
detected detected
regionalExpression detected in the dorsal neural tube in a Rhombomere specific pattern and the pharyngeal/caudal mandibular ectoderm.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ reporter
Entity Detected:Nog, noggin ( MGI:104327)
Notes:Nog expression was detected in this study by Stottmann et al., 2001 [PMID:11784076] as previously described by McMahon et al., 1998 [PMID:9585504] thus: The Noggin gene replacement construct was generated by blunt end ligation of a 5', 5.2-kb, EcoRV-BstEII Noggin genomic fragment into an end-filled (T4 polymerase) NcoI site in the lacZ containing plasmid, pSDKlacZ (the gift of Janet Rossant). This resulted in an in-frame fusion of the first 10 amino acids of the Noggin coding sequence with the E. coli lacZ gene containing an SV40 polyadenylation sequence. See reference [PMID:9585504] for more details.
Labelled with:LacZ
Specimen
Organism:mouse
Strain:either: (involves: 129S/SvEv * 129S1/Sv) or (involves: 129S1/Sv * C57BL/6)
Age:9.5 dpc
Theiler Stage:TS15
Mutations:Nogtm1Amc/Nog+
Preparation:wholemount
Procedures
General Information
Authors:Stottmann RW; Anderson RM; Klingensmith J, 2001 [PMID:11784076] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0479] [ PMID:11784076] Stottmann RW, Anderson RM, Klingensmith J 2001 The BMP antagonists Chordin and Noggin have essential but redundant roles in mouse mandibular outgrowth. Dev Biol (240):457-73
 [ doi:10.1101/gad.12.10.1438] [ PMID:9585504] McMahon JA, Takada S, Zimmerman LB, Fan CM, Harland RM, McMahon AP 1998 Noggin-mediated antagonism of BMP signaling is required for growth and patterning of the neural tube and somite. Genes Dev (12):1438-52
Links:MGI:3583825 same experiment
 MGI:1862000 allele description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI