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EMAGE:4092

Pitx2 paired-like homeodomain transcription factor 2 ( MGI:109340)
TS16 (10.0 dpc)
in situ hybridisation

Data Images
EMAGE:4092 EMAGE:4092
Fig5B. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.0133] Dev Biol 231: 252-64, Campione M; Ros MA; Icardo JM; Piedra E; Christoffels VM; Schweickert A; Blum M; Franco D; Moorman AF, Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. Copyright 2001 Fig5C. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2000.0133] Dev Biol 231: 252-64, Campione M; Ros MA; Icardo JM; Piedra E; Christoffels VM; Schweickert A; Blum M; Franco D; Moorman AF, Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. Copyright 2001

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Fig5C is a ventral view of the embryonic heart shown in a lateral view in Fig5B. Image annotations: la - left atrium; lv - left ventricle; oft - outflow tract; rv - right ventricle; ra - right atrium.
Expression Pattern Description
Spatial Annotation:
EMAGE:4092Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4092_wholemount_strong_3D_1.wlz
4092_wholemount_moderate_3D_1.wlz
4092_wholemount_weak_3D_1.wlz
4092_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4092_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Text Annotation:
StructureLevelPatternNotes
primitive ventricle
detected detected
regionalExpression is present in the ventral part of the right ventricle.
common atrial chamber right part
not detected not detected
homogeneous
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1330045
Entity Detected:Pitx2, paired-like homeodomain transcription factor 2 ( MGI:109340)
Notes:The Pitx2 probe used in this study by Campione et al., 2001 [PMID:11180966] was previously described by Campione et al., 1999 [PMID:10021341] as follows: mouse Pitx2 (1.7 kb XhoI-EcoRI fragment corresponding to entire coding sequence plus 5' and 3' UTR).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:FVB
Age:10.0 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Campione M; Ros MA; Icardo JM; Piedra E; Christoffels VM; Schweickert A; Blum M; Franco D; Moorman AF, 2001 [PMID:11180966] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2000.0133] [ PMID:11180966] Campione M, Ros MA, Icardo JM, Piedra E, Christoffels VM, Schweickert A, Blum M, Franco D, Moorman AF 2001 Pitx2 expression defines a left cardiac lineage of cells: evidence for atrial and ventricular molecular isomerism in the iv/iv mice. Dev Biol (231):252-64
 [ PMID:10021341] Campione M, Steinbeisser H, Schweickert A, Deissler K, van Bebber F, Lowe LA, Nowotschin S, Viebahn C, Haffter P, Kuehn MR, Blum M 1999 The homeobox gene Pitx2: mediator of asymmetric left-right signaling in vertebrate heart and gut looping. Development (126):1225-34
Links:MGI:3530358 same experiment
 EMAGE:4091 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI