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EMAGE:4110

Prkd3 protein kinase D3 ( MGI:1922542)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4110
Figure 1G. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.09.006] Gene Expr Patterns 6: 400-8, Oster H; Abraham D; Leitges M, Expression of the protein kinase D (PKD) family during mouse embryogenesis. Copyright 2006.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Ba, branchial arches; Fb, forebrain; Flb, forelimb bud; Mb, midbrain.
Expression Pattern Description
Spatial Annotation:
EMAGE:4110Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4110_wholemount_strong_3D_1.wlz
4110_wholemount_moderate_3D_1.wlz
4110_wholemount_weak_3D_1.wlz
4110_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4110_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
future forebrain
moderate moderate
future midbrain
moderate moderate
branchial arch
detected detected
limb
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3620201
Entity Detected:Prkd3, protein kinase D3 ( MGI:1922542)
Sequence:sense strand is shown

>MGI:3620201
ATCTGGCTACCAGTATCTCCGTGTCTAACTGTCAGGTTCAGGAGAATGTCGACATCAGCAGCGTTTACCA
GATCTTTGCGGACGAGGTGCTTGGTTCTGGCCAGTTTGGCATTGTTTATGGAGGAAAACACAGAAAGACG
GGAAGGGATGTGGCTATTAAAGTGATTGATAAAATGAGGTTCCCCACAAAGCAAGAGAGTCAGCTCCGGA
ACGAAGTGGCCATTTTACAGAATCTGCACCATCCTGGGATTGTAAACCTGGAGTGTATGTTTGAAACCCC
AGAACGAGTCTTCGTGGTGATGGAAAAGCTCCATGGAGATATGTTGGAAATGATTCTGTCCAGTGAAAAG
AGTCGACTTCCAGAACGGATTACCAAATTCATGGTCACACAGATACTTGTTGCCTTGAGGAATTTACATT
TCAAGAATATTGTGCACTGTGATTTAAAGCCCGAAAATGTGCTGCTTGCATCCGCAGAGCCATTTCCTCA
GGTGAAGCTGTGTGACTTTGGGTTTGCCCGCATCATTGGTGAGAAGTCGTTCCGGAGGTCAGTGGTAGGA
ACTCCAGCCTACTTAGCCCCTGAGGTTCTCAGGAGCAAAGGCTACAACCGTTCTCTAGACATGTGGTCGG
nt 2058 - nt 2687 of NM_029239.2
Notes:The Prkcn probe used in this study by Oster et al., 2006 [PMID:16377259] was generated using "primers for PKD3 [Prkcn] (GenBank accession number NM_029239; 5'-ATCTGGCTACCAGTATCTCCGT-3' and 5'-CCGACCACATGTCTAGAGAACG-3')" which targeted "the region 5' and including the first part of the kinase region of the corresponding transcripts".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + Purple AP Substrate
General Information
Authors:Oster H; Abraham D; Leitges M, 2006 [PMID:16377259] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/j.modgep.2005.09.006] [ PMID:16377259] Oster H, Abraham D, Leitges M 2006 Expression of the protein kinase D (PKD) family during mouse embryogenesis. Gene Expr Patterns (6):400-8
Links:MGI:3620459 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI