EMAGE mouse gene expression spatial database

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EMAGE:4124

Prkd2 protein kinase D2 ( MGI:2141917)
TS15 (9.5 dpc)
in situ hybridisation

Data Images


Figure 1E. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/j.modgep.2005.09.006] Gene Expr Patterns 6: 400-8, Oster H; Abraham D; Leitges M, Expression of the protein kinase D (PKD) family during mouse embryogenesis. Copyright 2006.

Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Notes:

Note: Ba, branchial arches; Flb, forelimb bud. The authors state that expression is absent in this specimen, which is in concordance with RT-PCR data. Some weak apparent staining was seen in the forelimb bud and the mandibular branchial arch, but this apparent signal was not distinguishable from that observed in embryos hybridized with the corresponding sense probe.

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

4124_wholemount_notDetected_3D_1.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:4124_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

not detected

homogeneous

Annotation Validation:

EMAGE Editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	MGI:3620202
Entity Detected:	Prkd2, protein kinase D2 ( MGI:2141917)
Sequence:	sense strand is shown >MGI:3620202 GCTCTCACTGTGCACTCGTACCGCGCTCCCGCCTTCTGTGACCACTGCGGGGAGATGCTCTTCGGCCTCG TGCGCCAGGGCCTCAAGTGCGATGGCTGCGGGCTGAATTACCACAAACGCTGCGCCTTCAGCATCCCGAA CAACTGCAGTGGTGCTCGAAAGCGCCGCCTGTCATCCACGTCTCTGGCCAGTGGCCATTCTGTGCGCCTT GGCAGCTCCGAGTCCTTACCCTGCACCGCCGAAGAGCTGAGCCGTAGCACCACCGACCTCCTTCCTCGCC GACCACCCTCGTCGTCGTCCTCCTCCTCTTCCTCTTCCTTCTACACAGGCCGACCCATTGAGTTGGACAA GATGCTGATGTCCAAGGTCAAGGTGCCACACACCTTCCTTATACATAGCTACACACGGCCCACCGTTTGC CAGGCTTGCAAGAAACTGCTCAAGGGTCTATTCCGCCAGGGCCTGCAGTGCAAAGACTGCAAGTTTAACT GTCACAAACGCTGTGCTACCCGCGTCCCTAACGACTGCCTGGGGGAGGCGCTCATCAATGGAGACGTGCC GATGGAGGAAGCCGCTGATTACAGTGAGGCTGATAAGAGCTCCATCTCAGATGAGTTGGAGGATTCTGGT GTCA
	nt 978 - nt 1611 of NM_178900.2
Notes:	The Prkd2 (PKD2) probe used in this study by Oster et al., 2006 [PMID:16377259] was generated using primers: "PKD2 (GenBank accession number NM_178900; 5'-GCTCTCACTGTGCACTCGTACC-3' and 5'-TGACACCAGAATCCTCCAACTG-3')...directed against the C1 and C1/PH regions".
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	9.5 dpc
Theiler Stage:	TS15
Mutations:	none (wild-type)
Preparation:	wholemount

Procedures

Fixation:

4% paraformaldehyde

General Information

Authors:	Oster H; Abraham D; Leitges M, 2006 [PMID:16377259] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	non-screen
References:	[ doi:10.1016/j.modgep.2005.09.006] [ PMID:16377259] Oster H, Abraham D, Leitges M 2006 Expression of the protein kinase D (PKD) family during mouse embryogenesis. Gene Expr Patterns (6):400-8
Links:	MGI:3620457 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source