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EMAGE:4151

Aplnr apelin receptor ( MGI:1346086)
TS16 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4151
Figure 2C. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(99)00081-7] Mech Dev 84: 199-203, Devic E; Rizzoti K; Bodin S; Knibiehler B; Audigier Y, Amino acid sequence and embryonic expression of msr/apj, the mouse homolog of Xenopus X-msr and human APJ. Copyright 1999.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: BA1, first branchial arch; FLB, forelimb buds; PCV, posterior cardinal veins; ISV, intersomitic vessels; LLCV, umen of the left cardinal vein; ACV, anterior cardinal vein.
Expression Pattern Description
Spatial Annotation:
EMAGE:4151Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4151_wholemount_strong_3D_1.wlz
4151_wholemount_moderate_3D_1.wlz
4151_wholemount_possible_3D_1.wlz
4151_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4151_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
1st branchial arch
strong strong
forelimb bud
strong strong
future forebrain
strong strong
arterial system
detected detected
Expression in the forming large vessels, brain vessels, and extending into the branchial arches, and intersomitic vessels.
venous system
detected detected
Expression in the forming large vessels, brain vessels, and extending into the branchial arches, and intersomitic vessels.
heart
weak weak
gut
weak weak
Expression in the endothelial lining of the left cardinal vein.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2450264
Entity Detected:Aplnr, apelin receptor ( MGI:1346086)
Sequence:sense strand is shown

>MGI:2450264
GGAAGGACTCTAAGCACCCTCAGACCTCTTACTCTGAGGTTCAAGCCAATCCTTCAGGGTGCTTGCTGAG
TTGGTGACCCATCTCTGGAACTGGTGTCCCTGGGACTCACTGCCCAGCTTCACTTCATTCAGCACCATGG
AAGATGATGGTTACAACTACTATGGGGCTGACAACCAGTCTGAATGCGACTACGCAGACTGGAAGCCCTC
TGGAGCGCTCATTCCTGCCATCTACATGTTGGTTTTTCTTCTAGGCACCACAGGCAATGGCCTGGTGCTC
TGGACCGTGTTTCGAACCAGCCGCGAAAAGAGACGCTCAGCTGACATCTTCATTGCCAGCCTGGCAGTGG
CTGACTTGACCTTTGTGGTGACTTTGCCACTGTGGGCCACTTATACCTACCGGGAGTTTGACTGGCCTTT
TGGAACCTTCTCTTGCAAGCTCAGCAGCTACCTCATCTTTGTCAACATGTACGCCAGTGTCTTTTGCCTC
ACCGGCCTCAGCTTTGACCGATACCTGGCCATTGTCAGGCCGGTGGCCAATGCTCGGCTAAGGCTGCGAG
TCAGCGGGGCCGTGGCCACAGCAGTCTTATGGGTGCTGGCTGCCCTTCTAGCTGTGCCTGTCATGGTGTT
CCGTTCCACAGACGCCTCGGAAAATGGCACCAAGATCCAGTGCTACATGGACTACTCTATGGTGGCCACT
TCAAACTCAGAGTGGGCCTGGGAGGTGGGCCTTGGGGTGTCCTCCACTGCCGTGGGCTTTGTGGTGCCCT
TCACCATCATGCTGACATGTTACTTCTTCATTGCCCAAACCATCGCTGGCCATTTCCGAAAGGAGCGCAT
TGAGGGCCTGCGGAAGAGGCGCCGGCTGCTCAGCATTATCGTGGTGCTTGTAGTGACCTTTGCCCTGTGC
TGGATGCCTTACCACCTGGTGAAGACTCTCTACATGCTGGGCAGTTTGCTGCACTGGCCCTGTGACTTTG
ACATCTTCCTCATGAATGTCTTTCCGTACTGCACCTGCATCAGTTATGTCAACAGCTGCCTCAACCCCTT
TCTCTATGCCTTCTTTGACCCCCGATTTCGCCAAGCCTGCACCTCCATGCTCTGCTGTGATCAGAGCGGG
TGCAAAGGCACCCCTCACAGCAGTAGTGCCGAGAAGTCAGCCAGTTATTCTTCCGGGCACAGCCAGGGCC
CTGGCCCCAACATGGGAAAGGGAGGAGAGCAGATGCATGAGAAATCGATTCCCTATAGTCAAGAAACCCT
TGTGGACTAG
nt 107 - nt 1376 of NM_011784.2
Notes:The Agtrl1 probe used in this study by Devic et al., 1999 [PMID:10473142] corresponded to "to 136 nucleotides in the 5' untranslated region and the entire coding sequence of msr/apj [Agtrl1] mRNA". Editors note: The co-ordinates with respect to the mouse Agtrl1 mRNA RefSeq NM_011784.2 above were deduced using this information.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:9.5 dpc
Theiler Stage:TS16
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Devic E; Rizzoti K; Bodin S; Knibiehler B; Audigier Y, 1999 [PMID:10473142] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(99)00081-7] [ PMID:10473142] Devic E, Rizzoti K, Bodin S, Knibiehler B, Audigier Y 1999 Amino acid sequence and embryonic expression of msr/apj, the mouse homolog of Xenopus X-msr and human APJ. Mech Dev (84):199-203
Links:MGI:2450268 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI