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EMAGE:4172

Nespas neuroendocrine secretory protein antisense ( MGI:1861674)
TS18 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:4172
Figure 1D. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(00)00490-1] Mech Dev 100: 79-81, Ball ST; Williamson CM; Hayes C; Hacker T; Peters J, The spatial and temporal expression pattern of Nesp and its antisense Nespas, in mid-gestation mouse embryos. Copyright 2001.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:4172Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4172_wholemount_strong_3D_1.wlz
4172_wholemount_moderate_3D_1.wlz
4172_wholemount_weak_3D_1.wlz
4172_wholemount_possible_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4172_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
trunk somite
weak weak
tail somite
weak weak
embryo
strong strong
regionalExpression is in the caudal portion of the embryo.
limb
detected detected
regionalExpression is in a crescent shape from its anterodistal to posterodistal margins.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:3033080
Entity Detected:Nespas, neuroendocrine secretory protein antisense ( MGI:1861674)
Sequence:sense strand is shown

>MGI:3033080
CGGCAGAAGTCCGCGCAACTTTATAGGGCCCCATTGCCCCATCTCTCCTCAGTCTCCTCTATCCTCTCCC
AGACGAGAGGATCAGTGGAGGCACCTCTCGGAGTCTTAGACTTCAGAGTCTGAGACTTAGCGAGAGGAGC
CTCGAGGAGACTCCTTCTCTCTTCTTTACCCATCCCTTTCTTTTACTTACAGCCACAAGCTGAGGCGCGG
AGCTTTAGAAAGTTCGCAGTGGTTTGAAGGTAGGTACATCCCTTCTTTTTGCCCCGAGGCGAGATTGGGG
TCTTCAGACCCCAGCTTCTCTCCTCATAAGGTTAATCCTTTTCGGCCCCAACAGTCCTTGCGCAGTGGGG
CCACTCTCTGCAGAGCCAGAGGGTGAGTCGGCTTCTCGGTGAGCACCTAAGAGAATGGATCGCAGGTCCC
G
nt 1323 - nt 1743 of AJ245401.1
Notes:The Nespas probe used in this study by Ball et al., 2001 [PMID:11118888] spanned "bases 1323-1743 of Nesp (accession no. AJ245401.1, Kelsey et al., 1999)".
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: 101/H * C3H/HeH
Age:10.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
General Information
Authors:Ball ST; Williamson CM; Hayes C; Hacker T; Peters J, 2001 [PMID:11118888] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(00)00490-1] [ PMID:11118888] Ball ST, Williamson CM, Hayes C, Hacker T, Peters J 2001 The spatial and temporal expression pattern of Nesp and its antisense Nespas, in mid-gestation mouse embryos. Mech Dev (100):79-81
Links:MGI:3033082 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI