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EMAGE:4180

Ifitm1 interferon induced transmembrane protein 1 ( MGI:1915963)
TS15 (9.5 dpc)
in situ hybridisation

Data Images
EMAGE:4180
Figure 6J. Copyright: Reprinted with permission from Elsevier from [doi:10.1016/S0925-4773(03)00126-6] Mech Dev 119 Suppl 1: S261-7, Tanaka SS; Matsui Y, Developmentally regulated expression of mil-1 and mil-2, mouse interferon-induced transmembrane protein like genes, during formation and differentiation of primordial germ cells. Copyright 2002.

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Note: Arrow indicates PGCs. Scale bar: 200 microns. ba, branchial arch; en, endothelium of the dorsal aorta; fl, fetal liver; tb, tail bud.
Expression Pattern Description
Spatial Annotation:
EMAGE:4180Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4180_wholemount_strong_3D_1.wlz
4180_wholemount_moderate_3D_1.wlz
4180_wholemount_possible_3D_1.wlz
4180_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4180_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
primordial germ cell
detected detected
foregut-midgut junction
detected detected
regionalExpression in the fetal liver.
dorsal aorta
detected detected
regionalExpression in the endothelium.
tail
detected detected
branchial arch
detected detected
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2664246
Entity Detected:Ifitm1, interferon induced transmembrane protein 1 ( MGI:1915963)
Sequence:sense strand is shown

>MGI:2664246
CCCTGTTCTTCACCATCCTCACGGCCATCGTCGTCATCGTTGTCTGTGCCATTAGATGATGTGAGATGTC
TTGCAACATCTCACAGTAGATAACAGATTCTGGGGCCTCCCAGGCTTGCTATGTGTTTCCTTGTCTATCG
CTGCCCCAAACCCTAGACTTAGTCCTGACCATTTGCCCCATACATATGCAAATGTGACACTCACAAATCT
GTCCATGGTGGACTCAATAAAGTGCACGTGCTGTG
nt 306 - nt 550 of NM_026820.1
Notes:The Ifitm1 (Mil-2) probe used in this study by Tanaka & Matsui, 2002 [PMID:14516695] was 244 bp and specific for Ifitm1. See also Figure 1A therein.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:involves: C57BL/6 * DBA/2 * ICR
Age:9.5 dpc
Theiler Stage:TS15
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Tanaka SS & Matsui Y, 2002 [PMID:14516695] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1016/S0925-4773(03)00126-6] [ PMID:14516695] Tanaka SS; Matsui Y 2002 Developmentally regulated expression of mil-1 and mil-2, mouse interferon-induced transmembrane protein like genes, during formation and differentiation of primordial germ cells. Mech Dev (119 Suppl 1):S261-7
Links:MGI:2664272 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI