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EMAGE:4222

Nrarp Notch-regulated ankyrin repeat protein ( MGI:1914372)
TS14 (8.75 dpc)
in situ hybridisation

Data Images
EMAGE:4222
Fig4A. Copyright: Reprinted with permission from Elsevier from [doi:10.1006/dbio.2001.0408] Dev Biol 238: 110-9, Krebs LT; Deftos ML; Bevan MJ; Gridley T, The nrarp gene encodes an ankyrin-repeat protein that is transcriptionally regulated by the notch signaling pathway. Copyright 2001

Expression pattern clarity: two stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
Image annotations: The arrowhead indicates an anterior mesodermal stripe.
Expression Pattern Description
Spatial Annotation:
EMAGE:4222Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
4222_wholemount_strong_3D_1.wlz
4222_wholemount_moderate_3D_1.wlz
4222_wholemount_weak_3D_1.wlz
4222_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:4222_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
central nervous system
strong strong
regionalExpression is in most of the central nervous system.
trunk paraxial mesenchyme
strong strong
regionalThere is an anterior stripe and a more extensive posterior domain.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:2153517
Entity Detected:Nrarp, Notch-regulated ankyrin repeat protein ( MGI:1914372)
Notes:The Nrarp probe used in this study by Krebs et al., 2001 [PMID:11783997] is described as follows: "The Nrarp in situ probe consisted of a 1.1-kb EcoRI fragment, which contained 300 nucleotides encoding the carboxy-terminus of the protein and 800 nucleotides of the 3' untranslated region." Editor's note: The authors provide the GenBank ID for the sequence of Nrarp (AY046077.1), which is a composite of the sequence from the clone discussed above and sequence obtained from several EST reads. EcoRI cuts AY046077.1 only once (within the ORF and 224 nt from the stop codon). Ambiguous information prevents defining the exact probe sequence based on published information
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:8.75 dpc
Theiler Stage:TS14
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Krebs LT; Deftos ML; Bevan MJ; Gridley T, 2001 [PMID:11783997] , Indexed by GXD, Spatially Mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ doi:10.1006/dbio.2001.0408] [ PMID:11783997] Krebs LT, Deftos ML, Bevan MJ, Gridley T 2001 The Nrarp gene encodes an ankyrin-repeat protein that is transcriptionally regulated by the notch signaling pathway. Dev Biol (238):110-9
Links:MGI:2154092 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI